Proteomics

Dataset Information

132

Rb and the APC/C coactivator FZR1 determine CDK4/6-Cyclin D requirement in C. elegans and human cancer cells


ABSTRACT: Cyclin dependent kinase 4 and 6 (CDK4/6) in complex with D-type cyclins promote cell cycle entry, at least in part through phosphorylation of the retinoblastoma tumor suppressor protein (Rb). The CDK4/6-cyclin D-Rb pathway is commonly deregulated in human cancer, often through CDK4/6 or D-type cyclin overexpression, or inactivation of the CDK4/6 antagonist p16/CDKN2A. Importantly, a substantial fraction of cancers depend on continuous CDK4/6-cyclin D kinase activity and are sensitive to CDK4/6-specific inhibitors. Here, we investigate critical CDK4/6-cyclin D functions that may determine the sensitivity to CDK4/6 inhibitors, making use of the essential roles of CDK4/6 (CDK-4) and cyclin D (CYD-1) in the nematode C. elegans. In an unbiased screen, we found that simultaneous loss of C. elegans Rb (lin-35) and down-regulation of the APC/C substrate specificity factor FZR1/Cdh1 completely overcomes CDK-4/CYD-1 requirement. Furthermore, CDK-4/CYD-1 phosphorylates specific residues in the LIN-35 Rb spacer domain and FZR-1 N-terminus that correspond to inactivating phosphorylations of the human homologs. Thus, CDK-4/CYD-1 appears to promote cell cycle entry by antagonizing not only transcriptional repression by LIN-35 Rb but also protein degradation by APC/CFZR-1. Simultaneous knockdown of Rb and FZR1 in human breast cancer cells synergistically overcomes arrest by the CDK4/6-specific inhibitor PD 00332991. These results reveal APC/CFZR1 as a putative CDK4/6-Cyclin D target and important contributing factor in the response to CDK4/6-inhibitor treatment.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Dengue virus   Myxococcus xanthus DK 1622   cf. Chlorella sp.   Synechocystis sp. PCC 6803 substr. Kazusa   Burkholderia cenocepacia   Botrytis cinerea B05.10   Francisella tularensis subsp. holarctica FSC200   Syntrophus aciditrophicus SB   Nicotiana longiflora   Porphyromonas gingivalis W83   Human herpesvirus 5 strain Merlin   Francisella tularensis subsp. holarctica   Desulfovibrio ferrophilus   Apis mellifera   Histomonas meleagridis   Staphylococcus pseudintermedius   Picea likiangensis var. rubescens   wastewater metagenome   environmental samples   Minona ileanae   Dolichospermum circinale AWQC310F   Phaseolus vulgaris   rat gut metagenome   Synechococcus phage syn9   Candidatus Paraburkholderia kirkii   Thermochaetoides thermophila DSM 1495   Heloderma suspectum suspectum   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759   Madurella mycetomatis   Siphoviridae   Bothrops brazili   Thermus thermophilus   Camellia sinensis   Microcystis aeruginosa PCC 7806   Acholeplasma laidlawii PG-8A   Natronomonas pharaonis DSM 2160   Canis lupus x Canis lupus familiaris   Mycobacterium avium subsp. paratuberculosis K-10   environmental samples   Listeria monocytogenes EGD-e   Capra hircus   Austrolebias   Mycobacterium tuberculosis SP7   Zygaena filipendulae   Rhizophagus irregularis   Mengo virus   Luteibacter   hybrid   Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841   Nicotiana repanda   Vibrio alginolyticus   Human alphaherpesvirus 1 strain KOS   Tetrahymena thermophila SB210   Gorilla gorilla   Yersinia enterocolitica   Penicillium sp. B12   Parasynechococcus marenigrum WH 8102   Nicotiana glutinosa   Mycobacteroides abscessus   Pisolithus tinctorius Marx 270   Leishmania chagasi   Zea mays   Thermococcus gammatolerans   Cryptococcus neoformans var. grubii H99S   Sorangium cellulosum So ce56   Olavius algarvensis Delta 1 endosymbiont   Magnaporthe sp. MG05   Viridiplantae   Lithobius forficatus   environmental samples   unidentified   Acipenser transmontanus   Burkholderia pseudomallei K96243   Leishmania donovani Ld 2001   Peniophora incarnata   Lactococcus lactis   Quercus robur   Micrurus tener   Phytophthora capsici   Pyrococcus abyssi   Phytophthora pisi   Lymnaea stagnalis   Bungarus   Penaeus vannamei   Escherichia coli   Bacillus cytotoxicus NVH 391-98   Orycteropus afer   Cellvibrio japonicus Ueda107   Ctenopharyngodon idella   Malaclemys terrapin   Paramecium tetraurelia   Citrobacter rodentium   Passer domesticus domesticus   Human herpesvirus 4 type 1   Strongyloides venezuelensis   Caldanaerobacter subterraneus subsp. tengcongensis MB4   Saccharomyces cerevisiae   Heterosigma akashiwo   Acanthoscurria geniculata   Fasciola hepatica   Meyerozyma guilliermondii   Sciuridae   unidentified chimeric sequence   Bacillus amyloliquefaciens   Synechocystis sp. PCC 6803   Andrias davidianus   Staphylococcus aureus subsp. aureus USA300   Nicotiana noctiflora   Brassica oleracea var. italica   Aspergillus niger NRRL3   Mycolicibacterium smegmatis MC2 155   Homo sapiens subsp. 'Denisova'   Sorghum bicolor   Desulforamulus reducens MI-1   Mus musculus x Rattus norvegicus   Cyclotella cryptica   Murine leukemia virus   Fusarium graminearum PH-1   Pyrococcus furiosus   Nicotiana alata   Lysiphlebus fabarum   Bartonella quintana JK 31   Platichthys flesus   Aerococcus urinae NBRC 15544 = CCUG 36881   Bovine leukemia virus   Pyrus x bretschneideri   Physcomitrella   Anopheles stephensi   Leishmania infantum JPCM5   Shigella sonnei 53G   Calicophoron daubneyi   Phytophthora sojae   Rhodobacter capsulatus SB 1003   Anopheles culicifacies B   Staphylococcus aureus   Delonix regia   Daktulosphaira vitifoliae   Metarhizium robertsii   Fraxinus mandshurica   Pongo pygmaeus   Tamias striatus   Bartonella quintana   Morganella   Capsaspora owczarzaki   Mycobacterium tuberculosis H37Rv   Pogona vitticeps   Staphylococcus aureus subsp. aureus USA300_TCH1516   [Candida] auris   Klebsiella pneumoniae   Hepatitis B virus   Tannerella forsythia   Bos taurus   Candida parapsilosis CDC317   Vitis labrusca x Vitis riparia   Viola tricolor subsp. tricolor   Streptococcus pyogenes STAB901   Schistosoma mansoni   Escherichia coli K-12   Rhizobium cf. leguminosarum   Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150   Mycobacterium tuberculosis SP13   [Candida] glabrata CBS 138   Entamoeba histolytica HM-1:IMSS-A   Ensifer adhaerens   anaerobic digester metagenome   Sistrurus miliarius barbouri   Pseudomonas stutzeri   Agrobacterium tumefaciens   Salmonella enterica subsp. enterica serovar Typhi str. CT18   Candidatus Endoriftia persephone   Toxoplasma gondii ME49   Aspergillus niger   Pseudomonas putida   Gammarus roeselii   Cervus nippon hortulorum   Euzebya tangerina   Selaginella moellendorffii   Trypanosoma cruzi Dm28c   Paradendryphiella salina   Coxsackievirus B3   Diplazium maximum   Human immunodeficiency virus   Stenotrophomonas maltophilia   Corynebacterium glutamicum K051   Pseudomonas fluorescens SBW25   Latilactobacillus curvatus   Plasmodium knowlesi strain H   Geobacter sulfurreducens   environmental samples   Thalassiosira pseudonana CCMP1335   Bacteria   Actinoplanes sp. SE50/110   Halobacterium salinarum R1   Bacillus subtilis subsp. subtilis str. 168   Allexis   Mus musculus musculus x Mus musculus castaneus   Pseudoliparis swirei   Nitrospirillum amazonense CBAmc   Aspergillus flavus NRRL3357   Salmo sp.   Bacillus thuringiensis   Streptococcus equi subsp. equi   Pachycladon exile   Anguilla anguilla   Plasmodium falciparum FcB1/Columbia   Pedobacter sp.   Sturnus vulgaris   Herpes simplex virus unknown type   Trichoderma reesei RUT C-30   Novosphingobium nitrogenifigens   Ceuthophilus sp. Ceu_104_TX_San_Rattlesnake   Oncorhynchus mykiss   Escherichia coli O157:H7 str. TW14359   Oryctolagus cuniculus   uncultured marine microorganism   Tapirus terrestris   Tursiops truncatus   Crustacea   Aspergillus giganteus   Acinetobacter baumannii D1279779   Solanum lycopersicum x Solanum tuberosum   Pachycladon fastigiatum   Leishmania donovani   Galloanserae   Streptococcus agalactiae   Nephotettix cincticeps   Lilium   Armoracia rusticana   Zalophus californianus   Dolichospermum circinale AWQC131C   Rhodopseudomonas palustris   Aedes aegypti densovirus   Nicotiana x sanderae   Haemophilus haemolyticus   Streptococcus pyogenes   Mytilus edulis/galloprovincialis MCLGV-2007   Cytomegalovirus   Nicotiana benthamiana   Hendra virus horse/Australia/Hendra/1994   Olavius algarvensis Gamma 1 endosymbiont   Vairimorpha necatrix   Lactococcus lactis subsp. lactis Il1403   Leishmania braziliensis MHOM/BR/75/M2904   albimanus section   Eukaryota   Bacillus pseudomycoides DSM 12442   Loxodonta africana   Rhodococcus jostii RHA1   Bordetella parapertussis   Trichoplax sp. H2   Deinococcus radiodurans R1   Fusarium oxysporum f. sp. lycopersici 4287   Conus geographus   Mycobacterium tuberculosis H37Rv_2009   Lactococcus lactis subsp. cremoris UC509.9   [Ruminococcus] gnavus   Streptococcus pneumoniae   Rhodopseudomonas palustris CGA009   Wolbachia sp. wMelPop   Dactylorhiza   Strix varia   Aggregatibacter actinomycetemcomitans   Deinococcus radiodurans   Colinus virginianus   Nicotiana quadrivalvis   Volvariella volvacea V23   Megoura viciae   Clostridioides difficile   Bubalus bubalis   Populus yunnanensis   Mycoplasma feriruminatoris   Olavius algarvensis   Passiflora edulis   Micrurus fulvius   Streptococcus pneumoniae TIGR4   Paracoccidioides brasiliensis Pb18   Merluccius bilinearis   Phytophthora infestans   Yersinia pestis   Trichoderma harzianum CBS 226.95   Pachysolen tannophilus NRRL Y-2460   Chlamydia trachomatis D/UW-3/CX   Euglena gracilis   Chlorella sp. SAG 211-18   Ricinus communis   Clostridium tetani E88   Scheffersomyces stipitis   Phaeobacter sp. MED193   Sistrurus catenatus   Heterorhabditis megidis   Panopea generosa   Streptococcus dysgalactiae subsp. equisimilis SK1249   Trichechus manatus   rhizosphere soil bacteria ensemble   Plasmodium sp. pBT9   Ambystoma mexicanum   Mammuthus sp.   Trypanosoma cruzi   Oryctolagus sp. 'Rabbit_OD'   Mayaro virus (strain Brazil)   Fusarium graminearum GZ3639   Deltaproteobacteria bacterium RIFCSPLOWO2_12_FULL_50_11   Lobaria pulmonaria   Trifolium pratense subsp. pratense   Ophiophagus hannah   Euprymna scolopes   Ursus arctos arctos   Conus betulinus   Methanoculleus sp. 10   Tetrahymena thermophila   Shewanella oneidensis MR-1   Enchytraeus crypticus   Piaractus mesopotamicus   Bradyrhizobium japonicum   Schizophyllum commune H4-8   Ianthella basta   Oryza sativa Indica Group   Physcomitrium patens   Plasmodium yoelii yoelii   Shigella flexneri 2a str. 2457T   Anopheles culicifacies A   Fraxinus nigra   Yarrowia lipolytica   Toxoplasma gondii CtCo5   Cyprinus carpio   Taenia hydatigena   Chlorocebus aethiops   Bombyx mori   Rhizobium favelukesii   Burkholderia cenocepacia K56-2Valvano   Candida tropicalis   Fusarium oxysporum f. sp. cucumerinum   Blastococcus saxobsidens   Sus scrofa domesticus   Quercus suber   Passer hispaniolensis   Cleome angustifolia   Cynodon dactylon   Mycobacterium tuberculosis M24   Populus tomentosa   Pseudomonas putida BIRD-1   Aspergillus fumigatus   Catenibacterium   Bifidobacterium kashiwanohense PV20-2   Heliocidaris erythrogramma   Trichomonas gallinae   Larimichthys crocea   Haemophilus influenzae   Ovis aries   Ruminiclostridium cellulolyticum H10   Nematostella vectensis   Enterococcus faecalis   Candidatus Methylomirabilis oxyfera   Cupiennius salei   Phytophthora capsici LT1534   unclassified Marseilleviridae   Nanger dama mhorr   Roseobacter sp. MED193   Lachancea kluyveri   Tribolium castaneum   Pseudomonas sp.   Tuber magnatum   Pinus taeda   Tuber melanosporum Mel28   Brucella melitensis   Spondylus gaederopus   Sulfolobus islandicus REY15A   Nicotiana rustica   Equus sp.   Granulicatella adiacens ATCC 49175   Chlamydomonas reinhardtii   Trypanosoma cruzi strain CL Brener   Drosophila melanogaster   Roseburia intestinalis L1-82   Toxoplasma gondii RH   Escherichia coli O157:NM   Saccharomyces cerevisiae W303   Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821   Streptomyces coelicolor   Streptococcus pneumoniae R6   Bradyrhizobium diazoefficiens   Rhynchocyon petersi   Rhizorhabdus wittichii   Ixodes scapularis   Synechococcus sp. PCC 7002   Marthasterias glacialis   Piper nigrum   Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044   Emiliania   Naja ashei   Mycobacterium tuberculosis   metagenomes   Citrus sinensis   Bacillus mycoides DSM 2048   Carica papaya   Struthio camelus   Puccinellia tenuiflora   Bifidobacterium pseudolongum   Listeria monocytogenes serotype 4b str. F2365   Trichoderma reesei QM9414   Simulium damnosum s.l. Me1   Agkistrodon contortrix   Synechocystis sp. PCC 6803 substr. GT-I   Vitis hybrid cultivar   Spirochaeta sp. ELBA   Cryptococcus neoformans var. grubii H99W   Helicobacter pylori J99   Cervus elaphus elaphus   Shigella flexneri   Nicotiana benavidesii   Tetrahymena thermophila CU428   Bothrops leucurus   Mycolicibacterium smegmatis   Campylobacter concisus UNSWCD   Riptortus pedestris   Enterococcus mundtii CRL35   Mammut americanum   Apteronotus leptorhynchus   Mycobacterium tuberculosis SP10   Halobacterium salinarum   Giardia intestinalis   Bacillus cereus ATCC 10987   Coffea arabica   Bartonella henselae str. Houston-1   Tropilaelaps mercedesae   Komagataella pastoris   Olavius algarvensis spirochete endosymbiont   Pseudomonas composti   Viola tricolor   Clostridioides difficile R20291   Pteropus rodricensis   Bordetella pertussis Tohama I   soil bacterium TS1114R   Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14   Entamoeba histolytica   Mycoplasmopsis agalactiae   Afipia carboxidovorans   Chlorella vulgaris   Homo   Ixodes ricinus   Brachypodium distachyon   Pseudoalteromonas sp. D41   Galleria mellonella   Perkinsus marinus   Avicennia officinalis   Candidatus Margulisbacteria   Cecropia peltata   Acyrthosiphon pisum   Listeria monocytogenes HCC23   Vicia faba   Medicago truncatula   Francisella tularensis   Paramoeba perurans   Bison   Morchella importuna   Aspergillus niger CBS 513.88   Lacticaseibacillus rhamnosus GG   Sepioloidea lineolata   Streptococcus equi subsp. equi 4047   Crotalus horridus   Cicer arietinum   Zymomonas mobilis   Tuber albidum   Scophthalmus maximus   Labeo rohita   Pachycladon cheesemanii   Bemisia tabaci   Roseburia   Coxiella burnetii   Spiroplasma melliferum KC3   Bothrops neuwiedi   Rhipicephalus microplus   Flavobacterium columnare   Brachyspiraceae   Mycobacterium tuberculosis SP1   Boehmeria nivea   Trypanosoma   Cyclopes didactylus   Ophrys x arachnitiformis subsp. archipelagi   Strigomonas culicis   Brugia malayi   mouse gut metagenome   Mesocricetus auratus   Spirometra erinaceieuropaei   Listeria monocytogenes   Nicotiana africana   Prototheca zopfii   Neospora caninum Liverpool   Melioribacter roseus P3M-2   Vitis   Streptococcus uberis   Blumeria hordei DH14   Oryctolagus cuniculus algirus   Lineus longissimus   Candidatus Protochlamydia amoebophila UWE25   Aves   Ganoderma lingzhi   Cricetulus griseus   environmental samples   Shigella dysenteriae   Haemaphysalis longicornis   Acheta domesticus   Bradyrhizobium   Severe acute respiratory syndrome coronavirus 2   Meleagris gallopavo   Synechococcus sp. BL107   freshwater sediment metagenome   Xanthomonas campestris   Acinetobacter sp. PM-14   Schizosaccharomyces pombe OY26   Nephila clavipes   Pseudotsuga menziesii   Pseudomonas taiwanensis   H1N1 subtype   Burkholderia pseudomallei 1026b   Schizosaccharomyces pombe   Apis mellifera pomonella   Clostridioides difficile 630   Caryophyllales   Dentex dentex   Saccharomyces cerevisiae BY4741   Pteropus alecto   Eryx colubrinus loveridgei   Pisum sativum   Neisseria gonorrhoeae   Deinococcus deserti VCD115   Potentilla saundersiana   Thermoanaerobacter pseudethanolicus   Paucilactobacillus suebicus   Fagus grandifolia   Citrus maxima   Coxiella-like endosymbiont of Rhipicephalus sanguineus complex   Brachylophosaurus canadensis   Ciona intestinalis   Microcystis aeruginosa   Escherichia coli O157:H7 str. EDL933   Alternaria tomato   Candidatus Kuenenia stuttgartiensis   Oreochromis niloticus niloticus   Clavibacter michiganensis subsp. nebraskensis NCPPB 2581   Pseudomonas putida KT2440   Gnathostoma spinigerum   Streptococcus pyogenes M1 GAS   Scutigera coleoptrata   Angiostrongylus cantonensis   Coccinia grandis   Synechococcus cyanophage syn   Synechococcus elongatus PCC 7942 = FACHB-805   Thymallus thymallus   Thermoplasma acidophilum DSM 1728   Corynebacterium glutamicum ATCC 13032   Olea europaea   Streptomyces avidinii   Panthera leo   Human papillomavirus type 16   Podospora anserina S mat+   Pseudomonas aeruginosa PA14   Mycobacterium tuberculosis KZN 1435   Streptococcus pneumoniae SP14-BS69   Botrytis cinerea T4   Aggregatibacter actinomycetemcomitans D7S-1   Sinorhizobium meliloti BL225C   Neptuniibacter caesariensis   Pectobacterium carotovorum   Hydra vulgaris   Hyalella azteca   Candidatus Nitrospira inopinata   Aspergillus nidulans FGSC A4   Tetronarce californica   Macaca mulatta   Daphnia magna   Vibrio cholerae O1 biovar El Tor str. N16961   Paracoccus denitrificans PD1222   Salmonella enterica subsp. enterica serovar Typhimurium str. SDT1291   Glia   Helianthus annuus   Amblyomma americanum   Marseillevirus   Candida albicans   Listeria phage A118   Fusarium oxysporum Fo47   Glycine max   Daphnia pulex   Tequatrovirus T4   Microcystis aeruginosa NIES-843   Vitis riparia   Mollivirus sibericum   Syntrophomonas wolfei   Bothrops cotiara   Ectocarpus siliculosus   Plasmodium berghei ANKA   Coprococcus eutactus   Scolopendra morsitans   Manica rubida   Durusdinium trenchii   Pachycladon novaezelandiae   Tradescantia spathacea   Avian infectious bronchitis virus (strain Beaudette CK)   Phascolarctos cinereus   Hepatitis C virus genotype 1   Thermothelomyces thermophilus ATCC 42464   Cryptochiton stelleri   Rattus norvegicus   Dictyostelium discoideum AX4   Lingula anatina   Bacillus subtilis   Parhyale hawaiensis   Brassica napus   Leptospira interrogans   Clostridium perfringens   Beauveria bassiana ARSEF 2860   Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344   Datura stramonium   Hepatitis E virus type 1   Nostoc sp. PCC 7120 = FACHB-418   Chromatiales bacterium OalgGamma1   Hypsiglena jani   Mycobacterium tuberculosis SP22   Tuber indicum   Oenococcus oeni   Apis mellifera ligustica   Pan troglodytes   Ceratopteris richardii   Helicobacter pylori H13-1   Saccharomyces mikatae IFO 1815   Helicobacter pylori A45   Haloferax volcanii DS2   Liquorilactobacillus nagelii   Cupriavidus metallidurans   Crassostrea hongkongensis   Cassiopea xamachana   Aeromonas salmonicida subsp. salmonicida   Fragaria x ananassa   Klebsiella pneumoniae BIDMC 40   Trichoderma reesei QM6a   Aplysia californica   Pseudomonas fluorescens   Panicum virgatum   Anas platyrhynchos   Cavia porcellus   Cenchrus americanus   Crocosphaera subtropica ATCC 51142   Neisseria gonorrhoeae FA 1090   Clostridium autoethanogenum   Ictalurus punctatus   unclassified Candidatus Omnitrophica   Saccharina japonica   Staphylococcus epidermidis   Canis lupus familiaris   Corynebacterium diphtheriae bv. mitis str. ISS 3319   Actinidia chinensis var. chinensis   Bursaphelenchus xylophilus   Schistosoma haematobium   Sophora alopecuroides   Capsicum annuum   Halobacterium   Bothrops moojeni   Ascaris suum   Saccharum sp.   Dioscorea oppositifolia   Acinetobacter baumannii ACICU   Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10   Ornithodoros moubata   Andreprevotia sp. IGB-42   Oryctolagus cuniculus cuniculus   Mycoplasma pneumoniae   Butyrivibrio fibrisolvens   Kluyveromyces marxianus   Mycoplasma pneumoniae M129-B7   Gallus gallus   Strongylocentrotus purpuratus   Saccharum hybrid cultivar   Naja mandalayensis   Cyanophora paradoxa   Leishmania major   Brassica oleracea var. gemmifera   Hippopotamus amphibius   Parasteatoda tepidariorum   Thermococcus kodakarensis KOD1   Fusarium sp.   Aspergillus flavus   Liquorilactobacillus hordei   Chlamydia trachomatis 434/Bu   Human alphaherpesvirus 1 strain 17   Pseudomonas putida F1   Boiga irregularis   Saccharopolyspora erythraea   Nilaparvata lugens   Clostridioides difficile 050-P50-2011   Cowpox virus (Brighton Red)   Formosa sp. Hel3_A1_48   Vitis vinifera   Aspergillus terreus NIH2624   Bombus terrestris   Corynebacterium diphtheriae NCTC 13129   Harmonia axyridis   Human herpesvirus 2 strain 186   Pseudo-nitzschia delicatissima   Dehalococcoides mccartyi CG1   Ostreococcus tauri   Pseudomonas aeruginosa PAO1   Dokdonia sp. MED134   Ustilaginoidea virens   Gammarus fossarum   Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S   Crotalus durissus terrificus   Nicotiana paniculata   Aspergillus fumigatus Af293   Bicyclus anynana   Catalpa fargesii   Pyricularia oryzae B157   Vigna mungo   Rhizobium sp. Leaf68   Theobroma cacao   Nicotiana clevelandii   Streptomyces coelicolor A3(2)   Eulimnogammarus cyaneus   Bacillus thuringiensis serovar berliner ATCC 10792   Neospora caninum   Nicotiana plumbaginifolia   Homo sapiens   Olavius algarvensis sulfate-reducing endosymbiont   Chaetoceros sp.   Staphylococcus cohnii   Streptococcus pyogenes ABC020006030   Coxiella burnetii RSA 493   Sarcophilus harrisii   Sitobion avenae   Amaranthus tricolor   Nicotiana forgetiana   Human herpesvirus 5 strain AD169   Petunia x hybrida   Methanosarcina acetivorans   Staphylococcus aureus subsp. aureus NCTC 8325   Vibrio anguillarum   Nicotiana tabacum   Yersinia pseudotuberculosis   Burkholderia cenocepacia H111   Stylosanthes guianensis   Beta vulgaris subsp. vulgaris   Ovis   Quercus petraea   Eremothecium gossypii   Escherichia coli O157:H7   Kluyveromyces lactis NRRL Y-1140   Aspergillus aff. niger A2   Strawberry leafy fruit phytoplasma   Chlorocebus sabaeus   Archaea   Capsicum annuum var. annuum   Synechococcus elongatus   Sparus aurata   Solanum lycopersicum   Trypanosoma brucei brucei   Candidatus Midichloriaceae   Muvirus mu   uncultured Phormidium sp.   Glycine soja   Bacteroides caccae   Colobus guereza   Sarcoptes scabiei   Populus trichocarpa   Plasmodium falciparum 3D7   Toxoplasma gondii GT1   [Candida] glabrata   Coronavirus PREDICT CoV-2   Staphylococcus haemolyticus   Drosophila pseudoobscura   Nicotiana langsdorffii   Dasypogon diadema   Trypanosoma cruzi strain Esmeraldo   Caenorhabditis elegans   Populus tremula x Populus tremuloides   Geodermatophilus obscurus DSM 43160   Metopolophium dirhodum   Cervus nippon nippon   Alicycliphilus denitrificans BC   Actinomycetia   Clostridium saccharoperbutylacetonicum   Xenopus laevis   Desulfovibrio sp. G11   Palaemonetes sp. common grass shrimp associated circular virus   Escherichia coli str. K-12 substr. MG1655   Saccharomyces cerevisiae EC1118   Onchocerca ochengi   Teleogryllus oceanicus   Piscirickettsia salmonis AUSTRAL-005   Hordeum vulgare   Rhizoctonia solani AG-8 WAC10335   Bothrops insularis   Alligator mississippiensis   Bothrops jararacussu   Nelumbo nucifera   Brucella abortus   Nostoc punctiforme PCC 73102   Staphylococcus aureus subsp. aureus MRSA252   Staphylococcus sp. 1   Naja sagittifera   Tuber aestivum   Helicobacter pylori E48   Salmonella enterica subsp. enterica serovar Typhimurium str. A130   Nicotiana glauca   Alteromonadales   Polytomella parva   Olavius algarvensis Delta 4 endosymbiont   Drosophila   Lactobacillus johnsonii N6.2   Clostridium cellulovorans   Homo sapiens/Mus musculus xenograft   Oreochromis mossambicus   Myzus persicae   Rousettus aegyptiacus   Butyrivibrio proteoclasticus B316   Echinococcus granulosus   Oncorhynchus tshawytscha   Fungi   Schistocephalus solidus   Arabidopsis thaliana   Toxoplasma gondii type III   Malus domestica   Dinoroseobacter shibae DFL 12 = DSM 16493   Trypanosoma brucei   Acinetobacter baumannii   Neosciurus carolinensis   Nicotiana maritima   Sphingobium chlorophenolicum   Staphylococcus aureus subsp. aureus USA300_FPR3757   Chenopodium quinoa   Pseudotsuga menziesii var. menziesii   Streptococcus thermophilus LMD-9   Cladocopium goreaui   Gossypium   Rangifer tarandus   Trypanosoma brucei brucei TREU927   Picea abies   Sus scrofa   Rheum palmatum   Salmonella enterica subsp. enterica serovar Typhimurium str. D23580   Taenia solium   Craterostigmus tasmanianus   Strigamia maritima   Lentinus crinitus   Lactococcus lactis subsp. cremoris MG1363   Clostridium butyricum E4 str. BoNT E BL5262   Pinus pinaster   Steinernema carpocapsae   Lactiplantibacillus plantarum JDM1   Bacillus mycoides NBRC 101238 = DSM 11821   Trichinella britovi   Medicago sativa   Mytilus edulis   environmental samples   Dioscorea alata   Vespula vulgaris   Pseudomonas aeruginosa LESB58   Bacillus toyonensis BCT-7112   Asterias rubens   Candidatus Pelagibacter ubique   Pseudomonas pseudoalcaligenes CECT 5344   Francisella orientalis FNO12   Avena strigosa   Tarenaya hassleriana   Klebsiella pneumoniae JH1   Python molurus   Candida albicans SC5314   Agaricus bisporus   Planococcus halocryophilus Or1   Equus asinus   Rhodotorula toruloides   Leishmania mexicana   Rhodospirillum rubrum ATCC 11170   Rattus rattus   Solanum tuberosum   Dichomitus squalens   Norovirus   Firmicutes   Secale cereale   Schizosaccharomyces pombe DM3650   human metagenome   Gossypium hirsutum   Brassica oleracea var. botrytis   Pseudomonas syringae   Candidatus Accumulibacter   Oryza sativa   Rosaceae   Giardia intestinalis assemblage A   Daphnia   Saccostrea glomerata   Channa striata   Bacillus cereus ATCC 14579   Dictyostelia   Cloning vector pEGFP-C1   Moraxella catarrhalis   Beta vulgaris   Haptophyta   Cryptococcus neoformans var. grubii H99   Epstein-barr virus strain p3hr-1   Thalassiosira cf. oceanica   Quercus ilex   Saccharomyces cerevisiae YJM789   Haemonchus contortus   Petunia   Candidatus Accumulibacter phosphatis   Paenibacillus sp.   Faecalibacterium prausnitzii   Acanthoscurria gomesiana   Mariprofundus ferrooxydans   Synechocystis sp.   Nicotiana sylvestris   Aspergillus nidulans TN02A3   Filifactor alocis   Bombus terrestris audax   Pichia fermentans   Acanthamoeba castellanii   Naja naja   prokaryotic environmental samples   Nicotiana gossei   Synechococcus phage S-SSM7   Russula atropurpurea   Trypanosoma vivax Y486   Dictyostelium discoideum   Escherichia coli BL21(DE3)   Leishmania mexicana MHOM/GT/2001/U1103   Callinectes sapidus   Prochlorococcus marinus str. MIT 9312   Amphibalanus amphitrite   Pseudomonas aeruginosa   Vitis riparia x Vitis rupestris   Legionella pneumophila subsp. pneumophila str. Philadelphia 1   Streptococcus agalactiae FSL S3-026   Ophrys sphegodes   Ictidomys tridecemlineatus   Tremarctos ornatus   Sphingomonas sp. Leaf257   Lactobacillus   Thermochaetoides thermophila   Agkistrodon piscivorus   Human alphaherpesvirus 1   Trichoderma sp. 1 YW-2011   Thermococcus gammatolerans EJ3   Mytilus sp. CHGF4   Borreliella burgdorferi B31   Salmonella enterica subsp. enterica serovar Typhimurium str. 5579   Neisseria meningitidis FAM18   Francisella tularensis subsp. holarctica LVS   Giardia lamblia ATCC 50803   Gadus morhua   Mycobacterium marinum ATCC BAA-535   Mylodon darwinii   sediment metagenome   Crassostrea gigas   Aspergillus nidulans   Rhodnius prolixus   Gammarus pulex   Eubacterium limosum   Microbiota   Lotus japonicus   Influenza A virus (H1N1)   Anopheles gambiae str. PEST   Spiroplasma melliferum   Pomacea canaliculata   Amphora coffeiformis   Bradyrhizobium diazoefficiens USDA 110   Mentha spicata   Methylocystis sp. SC2   Haloferax volcanii   Salmo salar   Escherichia coli Nissle 1917   Sedum plumbizincicola   Corucia zebrata   Moringa oleifera   Toxoplasma gondii   Streptococcus pneumoniae D39   Komagataella phaffii   Arachis hypogaea   Gorilla   Dialister   Malassezia sympodialis ATCC 42132   Craterostigma plantagineum   Cutibacterium acnes SK137   Mus musculus   Neisseria meningitidis MC58   Schizosaccharomyces pombe 927   Campylobacter concisus 13826   Helicobacter pylori 26695-1   Acinetobacter baumannii AB307-0294   Modestobacter marinus BC501   Clostridioides difficile ATCC 43255   Breviolum minutum   Salmonella enterica subsp. enterica serovar Enteritidis str. P125109   Pseudomonas aeruginosa LESlike1   Candidatus Thioglobus autotrophicus   Mustela putorius furo   Thermus thermophilus HB8   Medicago truncatula var. truncatula   Pinus radiata   uncultured bacterium   Hominidae   Bodo saltans   Flavobacterium sp. 316   Francisella tularensis subsp. novicida U112   Mus musculus wagneri   Danio rerio   Exiguobacterium sp. SH31   Coffea canephora   Brucella   gut metagenome   Staphylococcus aureus subsp. aureus str. Newman   Vibrio parahaemolyticus   Triticum aestivum   Cercopithecidae   Dendroaspis angusticeps   Equus asinus africanus   human gut metagenome   Oryza sativa Japonica Group   Sulfolobus acidocaldarius   Buchnera aphidicola (Acyrthosiphon pisum)   Latrodectus hesperus   Ustilago maydis   Mycoplasma pneumoniae M29   Diplonema papillatum   Emiliania huxleyi   Chelodina longicollis   Stappia stellulata DSM 5886   Phaeodactylum tricornutum CCAP 1055/1   Burkholderia thailandensis   Aeropyrum pernix K1   Arvicola   Peromyscus leucopus   Chlorobaculum tepidum   Neurospora crassa   Bothrops jararaca   Macaca fascicularis   Eriocheir sinensis   Panthera tigris   Viruses   Pituophis melanoleucus   Renibacterium salmoninarum ATCC 33209   Faustovirus   Lagomorpha   Mucilaginibacter   Plasmodium falciparum   Oryzias latipes   Human immunodeficiency virus 1   Oryza australiensis   Eucalyptus globulus   Taeniopygia guttata   Citrullus lanatus   Roseobacter denitrificans OCh 114   Populus tremula x Populus alba   Osphranter rufus   Anopheles gambiae   Odontotaenius disjunctus   Apostichopus japonicus   Nothobranchius furzeri   Shewanella baltica   Camelus dromedarius   Lachancea waltii NCYC 2644   Noccaea caerulescens   Felis catus   Nicotiana bonariensis   Xanthomonas oryzae pv. oryzae PXO99A   Gallus   (Vitis vinifera x Vitis berlandieri) x Vitis berlandieri   Coffea eugenioides   Octopodiformes   Pogonomyrmex rugosus   Enterobacter cloacae   Thermosynechococcus vestitus BP-1   Heligmosomoides polygyrus bakeri   Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499   Schmidtea mediterranea   Crotalus adamanteus   Gynandropsis gynandra   Ruminiclostridium papyrosolvens DSM 2782   Mycoplasma hyopneumoniae 232   Onchocerca volvulus   Phaeodactylum tricornutum   Pachycladon enysii   Musa acuminata subsp. acuminata   Leishmania major strain Friedlin   Oscillatoriaceae cyanobacterium   Lens culinaris   Eucalyptus   Aspergillus fumigatus CEA10   Pyricularia oryzae   Staphylococcus aureus subsp. aureus COL   Botrytis cinerea   Morganella   Mycobacterium tuberculosis TKK-01-0032   Tannerella forsythia 92A2   Elaeis guineensis   Crithidia fasciculata   Mytilus galloprovincialis   Photobacterium profundum SS9   Chlamydia trachomatis   biofilm metagenome   Pseudomonas aeruginosa PAO1H2O   Burkholderia sp. 2332   Mycoplasma gallisepticum   Schizosaccharomyces pombe 972h-   Mammalia   Dendroaspis polylepis polylepis   Diaphorina citri   Olavius algarvensis Gamma 3 endosymbiont   Prochlorococcus marinus str. MIT 9313   Ictalurid herpesvirus 1   Brucella abortus S19   Pyricularia oryzae P131   Brassica oleracea   Pseudovibrio sp. FO-BEG1   Nerodia clarkii   Dehalococcoides mccartyi   Klebsiella variicola At-22   Ictidomys tridecemlineatus arenicola   Ailuropoda melanoleuca   Lactococcus phage   Riftia pachyptila   Hebomoia glaucippe   Aedes aegypti aegypti   Dirofilaria immitis   Sanghuangporus   Acidithiobacillus ferrooxidans ATCC 53993   Stegodyphus mimosarum   Arabidopsis   Equus caballus   Yersinia pestis CO92   Clostridium phage c-st   Bacillus sp. 142311   Granulicella sp.   Priestia megaterium DSM 319   Ophrys garganica   Campylobacter jejuni   Listeria monocytogenes EGD   Vipera berus berus   Ficus carica   Helicobacter pylori 26695   Campylobacter fetus subsp. fetus 82-40   Frankia alni ACN14a   Marchantia polymorpha subsp. ruderalis   Globicatella sanguinis   Mycoplasma pneumoniae M129   Stephanorhinus   Mycoplasma gallisepticum S6   Bienertia sinuspersici   mixed culture metagenome   Burkholderia ubonensis MSMB22  

SUBMITTER: Alba Cristobal  

LAB HEAD: Albert J.R. Heck

PROVIDER: PXD001501 | Pride | 2016-07-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Celegans_1.msf Msf
Celegans_1.pep.xml Pepxml
Celegans_1.prot.xml Xml
Celegans_1A1.raw Raw
Celegans_1A2.raw Raw
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Publications


Cyclin-dependent kinases 4 and 6 (CDK4/6) in complex with D-type cyclins promote cell cycle entry. Most human cancers contain overactive CDK4/6-cyclin D, and CDK4/6-specific inhibitors are promising anti-cancer therapeutics. Here, we investigate the critical functions of CDK4/6-cyclin D kinases, starting from an unbiased screen in the nematode Caenorhabditis elegans. We found that simultaneous mutation of lin-35, a retinoblastoma (Rb)-related gene, and fzr-1, an orthologue to the APC/C co-activa  ...[more]

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