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Differentiation-inducing factor (DIF) SILAC

ABSTRACT: A global quantitative screen for phosphorylation changes that occur within the first minutes after addition of DIF-1 (differentiation inducing factor) to Dictyostelium cells, using a triple-label SILAC approach. Ax2 cells were labelled in light, medium and heavy medium then starved (5 hr) before DIF treatment. Cells were harvested at 1,5,8,15 minute post treatment to allow time-course of stimulation to be constructed from 2 overlapping triplex experimental samples A(0,1,8 min) amd B(0,5,15) with each experiment repeated in biological triplicate.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

SUBMITTER: Chris Sugden

LAB HEAD: JEFF WILLIAMS

PROVIDER: PXD001555 | Pride | 2015-01-28

REPOSITORIES: Pride

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The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes.

Sugden Chris C Urbaniak Michael D MD Araki Tsuyoshi T Williams Jeffrey G JG

Molecular biology of the cell 20141217 4

Differentiation-inducing factor-1 (DIF-1) is a polyketide that induces Dictyostelium amoebae to differentiate as prestalk cells. We performed a global quantitative screen for phosphorylation changes that occur within the first minutes after addition of DIF-1, using a triple-label SILAC approach. This revealed a new world of DIF-1-controlled signaling, with changes in components of the MAPK and protein kinase B signaling pathways, components of the actinomyosin cytoskeletal signaling networks, an ...[more]

PMID: 25518940

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