DYn-2 based identification of Arabidopsis sulfenomes

ABSTRACT: Identifying the sulfenylation state (-SOH) of stressed cells. Here, we optimized an in vivo trapping method of sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells. With click chemistry, the dimedone based alkyne functionalized DYn-2 probe was biotinylated for subsequent streptavidin enrichment of sulfenylated proteins. the DYn-2 modification and DYn-2-Biotin-azide modification were unknown modifications, we therefore chose N-D-glucuronylglycine [MOD00067] and N-palmitoyl-S-(sn-1-2,3-dipalmitoyl-glycerol)cysteine [MOD00444]

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell

SUBMITTER: Jarne Pauwels  

LAB HEAD: Kris Gevaert

PROVIDER: PXD001562 | Pride | 2016-06-27

REPOSITORIES: Pride