Proteomics

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Comparative proteomics of the ewe cerebrospinal fluid under long or short photoperiod


ABSTRACT: Photoperiod, the main physical synchronizer of seasonal functions, is now acknowledged as a key player for the modulation of molecule access to cerebrospinal fluid (CSF). From previous work showing a different transfer rate from steroids or protein hormones from blood to CSF according to photoperiod and, a modulation of choroid plexus (CP) tight junction protein content with photoperiod, we hypothesized that CSF proteome under long days (LD) would differ from that of CSF sampled under short days (SD) in ovariectomized-estradiol replaced ewes. CSF protein expression variability between SD- or LD-treated ewes was therefore studied from pools of CSF collected for 48H from ewes of similar age and weight.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Ovis Aries

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: G HARICHAUX  

LAB HEAD: Laurence Dufourny

PROVIDER: PXD001564 | Pride | 2015-06-09

REPOSITORIES: Pride

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Publications

Photoperiod affects the cerebrospinal fluid proteome: a comparison between short day- and long day-treated ewes.

Teixeira-Gomes A-P AP   Harichaux G G   Gennetay D D   Skipor J J   Thiery J-C JC   Labas V V   Dufourny L L  

Domestic animal endocrinology 20150428


Photoperiod is the main physical synchronizer of seasonal functions and a key factor in the modulation of molecule access to cerebrospinal fluid (CSF) in animals. Previous work has shown that photoperiod affects the transfer rate of steroids and protein hormones from blood to CSF and modulates choroid plexus tight junction protein content. We hypothesized that the CSF proteome would also be modified by photoperiod. We tested this hypothesis by comparing CSF obtained from the third ventricle of m  ...[more]

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