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CoIP of Nrl1 from normal and irradiated fission yeast cells

ABSTRACT: Nrl1-associated proteins were isolated from wild-type cells untreated or exposed to 100Gy of ionising radiation, and then subjected to LC-MSMS analysis. Identifications were carried out using trypsin as protease.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Schizosaccharomyces Pombe

SUBMITTER: Dorothea Anrather

LAB HEAD: Markus Hartl

PROVIDER: PXD001583 | Pride | 2016-01-04

REPOSITORIES: Pride

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The spliceosome-associated protein Nrl1 suppresses homologous recombination-dependent R-loop formation in fission yeast.

Aronica Lucia L Kasparek Torben T Ruchman David D Marquez Yamile Y Cipak Lubos L Cipakova Ingrid I Anrather Dorothea D Mikolaskova Barbora B Radtke Maximilian M Sarkar Sovan S Pai Chen-Chun CC Blaikley Elizabeth E Walker Carol C Shen Kuo-Fang KF Schroeder Renee R Barta Andrea A Forsburg Susan L SL Humphrey Timothy C TC

Nucleic acids research 20151217 4

The formation of RNA-DNA hybrids, referred to as R-loops, can promote genome instability and cancer development. Yet the mechanisms by which R-loops compromise genome instability are poorly understood. Here, we establish roles for the evolutionarily conserved Nrl1 protein in pre-mRNA splicing regulation, R-loop suppression and in maintaining genome stability. nrl1Δ mutants exhibit endogenous DNA damage, are sensitive to exogenous DNA damage, and have defects in homologous recombination (HR) repa ...[more]

PMID: 26682798

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