Proteome profiling of sea star epidermal secretions

ABSTRACT: Marine organisms represent a rich source for discovering natural products and materials that could inspire the development of novel molecules or materials for a high variety of industrial applications. Among them are sea stars, emblematic animals of the seashore. These organisms rely on epidermal secretions to cope with their benthic life. Their integument produces a mucus, which represents the first barrier against invaders; and their tube feet produce adhesive secretions to pry open mussels and attach strongly but temporarily to rocks. In this study, we investigate for the first time the protein content of mucous and adhesive secretions from the sea star Asterias rubens. These secretomes were analysed using tandem mass spectrometry and resulting MS/MS data were searched against in silico translated tube foot transcriptome. Tube foot transcripts coding for proteins identified in the two secretions were then functionally annotated by similarity searches against NCBI nr database.

INSTRUMENT(S):

ORGANISM(S): Asterias Rubens

SUBMITTER: Elise Hennebert  

LAB HEAD: Igor Eeckhaut

PROVIDER: PXD001607 | Pride | 2015-07-20

REPOSITORIES: Pride