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Roles of proteome dynamics and cytokinin signaling in root-to-hypocotyl ratio changes induced by shading roots of Arabidopsis seedlings

ABSTRACT: Seeds of Arabidopsis thaliana ecotype Columbia (Col-0) were surface-sterilized (using 75 % ethanol) and sown on Murashige and Skoog medium (pH 5.7) supplemented with 3 mM morpholinoethane sulfonic acid, solidified with 1% (w/v) agar and stratified at 4 °C for 48 h. Petri dishes were covered by a layer of black stretch shrink wrap film (black foil) and aluminum foil and incubated at 21°C/19°C day/night temperatures, with a 16 h photoperiod (photosynthetic photon fluence rate of 20 μmol m-2 s-1 over the waveband 400-700 nm) for 7 d in an AR-36L growth chamber

INSTRUMENT(S): maXis

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Whole Body

SUBMITTER: Martin Cerny

LAB HEAD: Martin Cerny

PROVIDER: PXD001612 | Pride | 2015-02-23

REPOSITORIES: Pride

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Roles of proteome dynamics and cytokinin signaling in root to hypocotyl ratio changes induced by shading roots of Arabidopsis seedlings.

Novák Jan J Černý Martin M Pavlů Jaroslav J Zemánková Jana J Skalák Jan J Plačková Lenka L Brzobohatý Břetislav B

Plant & cell physiology 20150219 5

In nature, root systems of most terrestrial plants are protected from light exposure by growing in a dark soil environment. Hence, in vitro cultivation in transparent Petri dishes leads to physiological perturbations, but the mechanisms underlying root-mediated light perception and responses have not been fully elucidated. Thus, we compared Arabidopsis thaliana seedling development in transparent and darkened Petri dishes at low light intensity (20 µmol m(-2) s(-1)), allowing us to follow (inter ...[more]

PMID: 25700275

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