Proteomics

Dataset Information

82

Roles of proteome dynamics and cytokinin signaling in root-to-hypocotyl ratio changes induced by shading roots of Arabidopsis seedlings


ABSTRACT: Seeds of Arabidopsis thaliana ecotype Columbia (Col-0) were surface-sterilized (using 75 % ethanol) and sown on Murashige and Skoog medium (pH 5.7) supplemented with 3 mM morpholinoethane sulfonic acid, solidified with 1% (w/v) agar and stratified at 4 °C for 48 h. Petri dishes were covered by a layer of black stretch shrink wrap film (black foil) and aluminum foil and incubated at 21°C/19°C day/night temperatures, with a 16 h photoperiod (photosynthetic photon fluence rate of 20 μmol m-2 s-1 over the waveband 400-700 nm) for 7 d in an AR-36L growth chamber

INSTRUMENT(S): maXis

ORGANISM(S): Arabidopsis thaliana  

TISSUE(S): Whole Body

DISEASE(S): Not Available

SUBMITTER: Martin Cerny  

LAB HEAD: Martin Cerny

PROVIDER: PXD001612 | Pride | 2015-02-23

REPOSITORIES: pride

altmetric image

Publications

Roles of proteome dynamics and cytokinin signaling in root to hypocotyl ratio changes induced by shading roots of Arabidopsis seedlings.

Novák Jan J   Černý Martin M   Pavlů Jaroslav J   Zemánková Jana J   Skalák Jan J   Plačková Lenka L   Brzobohatý Břetislav B  

Plant & cell physiology 20150219 5


In nature, root systems of most terrestrial plants are protected from light exposure by growing in a dark soil environment. Hence, in vitro cultivation in transparent Petri dishes leads to physiological perturbations, but the mechanisms underlying root-mediated light perception and responses have not been fully elucidated. Thus, we compared Arabidopsis thaliana seedling development in transparent and darkened Petri dishes at low light intensity (20 µmol m(-2) s(-1)), allowing us to follow (inter  ...[more]

Similar Datasets

2019-02-21 | PXD007678 | Pride
2007-03-10 | E-MEXP-574 | ArrayExpress
2012-09-25 | E-MEXP-3710 | ArrayExpress
2007-05-31 | GSE7942 | GEO
2006-08-23 | E-MEXP-411 | ArrayExpress
2015-05-26 | PXD001567 | Pride
| GSE88788 | GEO
2019-01-01 | S-EPMC6835955 | BioStudies
2019-01-01 | S-EPMC6682973 | BioStudies
2020-01-01 | S-EPMC7080443 | BioStudies