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Bacillus subtilis acetylome and succinylome

ABSTRACT: The two major acyl-modifications on lysine residues, acetylation and succinylation, were analyzed in the gram-positive model bacterium Bacillus subtilis. To identify and quantify the changes in acetylation and succinylation in response to the carbon source, a stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomic analysis of cells grown on glucose or citrate was performed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

TISSUE(S): Cell Lysate

SUBMITTER: Saori Kosono

LAB HEAD: Saori Kosono

PROVIDER: PXD001615 | Pride | 2015-07-02

REPOSITORIES: Pride

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Changes in the Acetylome and Succinylome of Bacillus subtilis in Response to Carbon Source.

Kosono Saori S Tamura Masaru M Suzuki Shota S Kawamura Yumi Y Yoshida Ayako A Nishiyama Makoto M Yoshida Minoru M

PloS one 20150622 6

Lysine residues can be post-translationally modified by various acyl modifications in bacteria and eukarya. Here, we showed that two major acyl modifications, acetylation and succinylation, were changed in response to the carbon source in the Gram-positive model bacterium Bacillus subtilis. Acetylation was more common when the cells were grown on glucose, glycerol, or pyruvate, whereas succinylation was upregulated when the cells were grown on citrate, reflecting the metabolic states that prefer ...[more]

PMID: 26098117

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