Dataset Information

Response to phosphate limitation of Pseudovibrio sp. FO-BEG1, ICPL-LC-MS/MS, Cytoplasmatic proteome

ABSTRACT: In this study we investigated the response of Pseudovibrio sp. FO-BEG1 to phosphate limitation. We compared the protein expression of strain FO-BEG1 under phosphate limited and phosphate surplus conditions. This dataset refers to the proteome analysis conducted on the cytoplasmatic proteins. An additional dataset for the extracellular and the membrane proteome is available. These two proteomes and the cytoplasmatic proteome were obtained from indipendent cultivation experiments.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Pseudovibrio Sp. (strain Fo-beg1)�

TISSUE(S): Prokaryotic Cell

SUBMITTER: Stefano Romano

LAB HEAD: Heide Schulz-Vogt

PROVIDER: PXD001624 | Pride | 2016-06-24

REPOSITORIES: Pride

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Publications

Phosphate Limitation Induces Drastic Physiological Changes, Virulence-Related Gene Expression, and Secondary Metabolite Production in Pseudovibrio sp. Strain FO-BEG1.

Romano Stefano S Schulz-Vogt Heide N HN Schulz-Vogt Heide N HN González José M JM Bondarev Vladimir V

Applied and environmental microbiology 20150313 10

Phosphorus is a vital nutrient for living organisms and is obtained by bacteria primarily via phosphate uptake. However, phosphate is often scarcely accessible in nature, and there is evidence that in many areas of the ocean, its concentration limits bacterial growth. Surprisingly, the phosphate starvation response has been extensively investigated in different model organisms (e.g., Escherichia coli), but there is a dearth of studies on heterotrophic marine bacteria. In this work, we describe t ...[more]

PMID: 25769826

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Project description:Total transcriptome analyses were carried out to investigate changes in transcript patterns in the Mycobacterium smegmatis wild type strain SMR5 due to nitrogen starvation and to expand the knowledge about the role of the two transcriptional regulators of nitrogen metabolism, namely GlnR and AmtR, in these processes. A first experiment revealed enhanced transcript levels of 284 genes and reduced transcripts of 231 genes in the wild type under nitrogen starvation compared to nitrogen surplus. When glnR deletion strain MH1 was compared to the wild type under nitrogen starvation, decreased transcript levels of 125 genes were detected, indicating that these are activated by GlnR due to nitrogen limitation. Comparing amtR deletion strain YL1 to the wild type under nitrogen starvation, enhanced transcript levels of 2 genes were found, indicating that they are repressed by AmtR under nitrogen surplus. A comparison of YL1 and wild type under surplus, as well as a comparison of YL1 under nitrogen surplus and starvation and of MH1 under nitrogen surplus and starvation were carried out as additional control experiments. It can be concluded that GlnR is the master regulator of nitrogen control in M. smegmatis and that AmtR fulfills only a small, subordinate role in the regulation of an operon. This SuperSeries is composed of the following subset Series: GSE30033: Mycobacterium smegmatis - Comparison of glnR deletion strain MH1 under nitrogen surplus and starvation GSE30231: Mycobacterium smegmatis - Comparison of wild type SMR5 and amtR deletion strain YL1 under nitrogen starvation GSE30232: Mycobacterium smegmatis - Comparison of amtR deletion strain YL1 under nitrogen surplus and starvation GSE30233: Mycobacterium smegmatis - Comparison of wild type SMR5 under nitrogen surplus and starvation GSE30234: Mycobacterium smegmatis - Comparison of wild type SMR5 and glnR deletion strain MH1 under nitrogen starvation GSE30235: Mycobacterium smegmatis - Comparison of wild type SMR5 and amtR deletion strain YL1 under nitrogen surplus Refer to individual Series

Dataset Information

Response to phosphate limitation of Pseudovibrio sp. FO-BEG1, ICPL-LC-MS/MS, Cytoplasmatic proteome

Publications

Phosphate Limitation Induces Drastic Physiological Changes, Virulence-Related Gene Expression, and Secondary Metabolite Production in Pseudovibrio sp. Strain FO-BEG1.

Similar Datasets

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