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Quantitative proteomic analysis of mouse hippocampus in the context of fear memory

ABSTRACT: Quantitative proteomic analysis was used to identify the proteins associated with a formation of fear memory in mice. The proteins from the hippocampal region were isolated from three groups of trained aminals representing aquisition, consolidation and retrieval of a contextual fear conditioning. The samples were digested by trypsin, analyzed by LC-MSMS followed by protein identification and label-free quantitation using MaxQuant 1.3.0.5. Each group consisted of three individuals and each sample was processed in two technical replicates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Roman Smidak

LAB HEAD: Gert Lubec

PROVIDER: PXD001639 | Pride | 2017-11-17

REPOSITORIES: Pride

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Quantitative proteomics reveals protein kinases and phosphatases in the individual phases of contextual fear conditioning in the C57BL/6J mouse.

Šmidák Roman R Mayer Rupert Laurenz RL Bileck Andrea A Gerner Christopher C Mechtcheriakova Diana D Stork Oliver O Lubec Gert G Li Lin L

Behavioural brain research 20151231

A series of protein kinases and phosphatases (PKPs) have been linked to contextual fear conditioning (cFC) but information is mainly derived from immunochemical studies. It was therefore decided to use an explorative label-free quantitative proteomics approach to concomitantly determine PKPs in hippocampi of mice in the individual phases of cFC. C57BL/6J mice were divided into four groups: three training groups representing the acquisition, consolidation and retrieval phases of cFC and a foot sh ...[more]

PMID: 26748257

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