HA-mSLX4 wild type and SIMdependent interactors

ABSTRACT: SUMOylation is known to play important roles in the DNA damage response, however, a role for SUMOylation in interstrand crosslink repair remains enigmatic. We report on the regulation of the SLX4 nuclease scaffold protein by SUMOylation. SLX4 contains three SUMO-Interaction Motifs (SIMs). Mutating all three SIMs abrogated the binding of SLX4 to SUMO-2 and covalent SLX4 SUMOylation. We attempt to identify which protein interaction are SIM dependent.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Román González-Prieto  

LAB HEAD: Alfred C O Vertegaal

PROVIDER: PXD001681 | Pride | 2015-03-02

REPOSITORIES: Pride