Fission yeast DUB substrates - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival

ABSTRACT: His-Biotin-His Ubiquitin was overexpressed in various fission yeast (S. pombe) genetic backgrounds to identify ubiqutinated proteins. Normalizing to bait (ubiquitin) across samples provided insight into relative changes in ubiquitination with loss of deubiquitinases (DUBs), yielding putative DUB substrates.

INSTRUMENT(S):

ORGANISM(S): Schizosaccharomyces Pombe

SUBMITTER: Janel Beckley  

LAB HEAD: Kathleen L. Gould

PROVIDER: PXD001767 | Pride | 2017-06-09

REPOSITORIES: Pride