Dataset Information

Heart Mitochondrial Proteome - The Failing Heart Relies on Ketone Bodies as a Fuel

ABSTRACT: Isolation of Heart Mitochondria of defined stages of pressure overload-induced heart failure in mice

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Marcus Krueger

LAB HEAD: Krueger

PROVIDER: PXD001820 | Pride | 2016-05-09

REPOSITORIES: Pride

ACCESS DATA

Publications

The Failing Heart Relies on Ketone Bodies as a Fuel.

Aubert Gregory G Martin Ola J OJ Horton Julie L JL Lai Ling L Vega Rick B RB Leone Teresa C TC Koves Timothy T Gardell Stephen J SJ Krüger Marcus M Hoppel Charles L CL Lewandowski E Douglas ED Crawford Peter A PA Muoio Deborah M DM Kelly Daniel P DP

Circulation 20160127 8

<h4>Background</h4>Significant evidence indicates that the failing heart is energy starved. During the development of heart failure, the capacity of the heart to utilize fatty acids, the chief fuel, is diminished. Identification of alternate pathways for myocardial fuel oxidation could unveil novel strategies to treat heart failure.<h4>Methods and results</h4>Quantitative mitochondrial proteomics was used to identify energy metabolic derangements that occur during the development of cardiac hype ...[more]

PMID: 26819376

Similar Datasets

Project description:Mechanical unloading by ventricular assist devices (VAD) leads to significant gene-expression changes often summarized as reverse remodeling. However, little is known on individual transcriptome changes during VAD-support and its relationship to non-failing hearts (NF). In addition no data are available for the transcriptome regulation during non-pulsatile VAD-support. Therefore we analysed the gene-expression patterns of 30 paired samples from VAD-supported (including 8 non-pulsatile VADs) and 8 non-failing control hearts (NF) using the first total human genome-array available. Transmural myocardial samples were collected for RNA-isolation. RNA was isolated by commercial methods and processed according to chip-manufacturer recommendations. cRNA were hybridized on Affymetrix HG-U133 Plus 2.0 arrays, providing coverage of the whole human genome Array. Data was analyzed using Microarray Analysis Suite 5.0 (Affymetrix) and clustered by Expressionist software (Genedata). 352 transcripts were differentially regulated between samples from VAD-implantation and NF, whereas 510 were significantly regulated between VAD-transplantation and NF (paired t-test p<0.001, fold change >=1.6). Remarkably, only a minor fraction of 111 transcripts was regulated in heart failure (HF) and during VAD-support. Unsupervised hierarchical clustering of paired VAD- and NF-samples revealed separation of HF- and NF- samples, however individual differentiation of VAD-implantation and VAD-transplantation was not accomplished. Clustering of pulsatile and non-pulsatile VAD did not lead to robust separation of gene expression patterns. During VAD-support myocardial gene expression changes do not indicate reversal of the HF-phenotype, but reveal a distinct HF-related pattern. Transcriptome analysis of pulsatile and non-pulsatile VAD-supported hearts did not provide evidence for a pump-mode specific transcriptome pattern. Microarrays were used to elucidate the differences between non-failing control hearts and those, suffering from end-stage heart failure pre and post mechanical unloading.

Dataset Information

Heart Mitochondrial Proteome - The Failing Heart Relies on Ketone Bodies as a Fuel

Publications

The Failing Heart Relies on Ketone Bodies as a Fuel.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure