Improved protease digestion conditions for membrane protein detection
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q TRAP
ORGANISM(S): Escherichia Coli
SUBMITTER: 
Lie Min
LAB HEAD: Kelvin H. Lee
PROVIDER: PXD001824 | Pride | 2015-04-28
REPOSITORIES: Pride
Publications
Improved protease digestion conditions for membrane protein detection.
Min Lie L Choe Leila H LH Lee Kelvin H KH
Electrophoresis 20150610 15
This work presents improved protease digestion conditions for membrane protein detection. The enzymatic digest of bacteriorhodopsin (BR), a model membrane protein with seven transmembrane domains (TMDs) was investigated. An initial in-gel digestion identified 17% BR sequence coverage, including part of the seventh TMD. To improve sequence coverage, BR digest was tested with different concentrations of RapiGest, methanol (MeOH) and SDS using either trypsin or chymotrypsin. Two improved conditions ...[more]
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