Browse
Submit Data
Databases
API
Help

Dataset Information

343 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Phosphorylation of Cohesin's Deposition Factor, Scc2 Regulates its Deposition Activity

ABSTRACT: Phosphorylated residues of the budding yeast Scc2 protein were identified using phosphopeptide enrichment and mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Matthew Wither

LAB HEAD: Paul C. Megee

PROVIDER: PXD001830 | Pride | 2015-09-10

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Phosphorylation of the Scc2 cohesin deposition complex subunit regulates chromosome condensation through cohesin integrity.

Woodman Julie J Hoffman Matthew M Dzieciatkowska Monika M Hansen Kirk C KC Megee Paul C PC

Molecular biology of the cell 20150909 21

The cohesion of replicated sister chromatids promotes chromosome biorientation, gene regulation, DNA repair, and chromosome condensation. Cohesion is mediated by cohesin, which is deposited on chromosomes by a separate conserved loading complex composed of Scc2 and Scc4 in Saccharomyces cerevisiae. Although it is known to be required, the role of Scc2/Scc4 in cohesin deposition remains enigmatic. Scc2 is a phosphoprotein, although the functions of phosphorylation in deposition are unknown. We id ...[more]

PMID: 26354421

Similar Datasets

Xanthine Oxidoreductase mediates milk fat globule secretion.

Project description:Analysis of the milk fat globule proteome from mice with a mammary-specific knockout of Xanthine Oxidoreductase.

2017-05-31 | PXD003336 | Pride

Characterization of seminal vesicle proteome of cactus field mouse (Peromyscus eremicus) in hydrated and dehydrated conditions

Project description:ABSTRACT: The cactus mouse (Peromyscus eremicus) is a desert-specialized rodent that experiences both chronic and acute dehydration in the Southwestern United States. Our previous research has generated substantial transcriptomic data on P. eremicus kidneys, testes, epididymis, and vas deferens in individuals exposed to hydrated and dehydrated conditions; however, the study described here is the first to describe a seminal vesicle proteome for this species. We have produced a seminal vesicle proteome from P. eremicus with free access to water and mice that were acutely dehydrated to generate a dataset that is comprehensive for both alternative water-availability states experienced by this species. We have also provided gene ontologies for this proteome using PANTHER. This proteome will provide a crucial resource for future studies characterizing the genetic and proteomic responses of reproductive tissues to drought in this rodent. Furthermore, an enhanced understanding of survival and reproductive responses (and adaptations) to dehydration is particularly relevant to clinical work aiming to minimize adverse human impacts as climate change continues to increase the incidence of drought.

2022-03-02 | PXD006532 | Pride

Cephalopod Chromatophore Proteomics

Project description:using the first annotated squid chromatophore proteome together with microscopy, we identified likely biochemical components of this reflective coloration as structural reflectin proteins distributed in cells that completely envelop each chromatocyte. Within the chromatocytes, where the pigment molecules reside in nanostructured granules, we found the lens protein Ω- crystallin.

2019-03-05 | PXD011975 | Pride

Characterization of R-loop-interacting proteins in embryonic stem cells reveals roles in ribosomal RNA processing and gene expression

Project description:Chromatin-associated RNAs have diverse roles in the nucleus. However, their mechanisms of action are poorly understood, in part due to the inability to identify proteins that specifically associate with chromatin-bound RNAs. Here, we address this problem for a subset of chromatin-associated RNAs that form R-loops—RNA-DNA hybrid structures that include a displaced strand of single-stranded DNA. R-loops generally form co-transcriptionally and have important roles in regulation of gene expression, immunoglobulin class switching, and other processes. However, unresolved R-loops can lead to DNA damage and chromosome instability. To identify factors that may bind and potentially regulate R-loop accumulation or mediate R-loop-dependent functions, we used a comparative immunoprecipitation/mass spectrometry approach, with and without RNA-protein crosslinking, to identify a stringent set of R-loop-binding proteins in mouse embryonic stem cells. We identified 365 R-loop-interacting proteins, which were highly enriched for proteins with predicted RNA-binding functions. We characterized several R-loop-interacting proteins of the DEAD-box family of RNA helicases and found that these proteins localize to the nucleolus and, to a lesser degree, the nucleus. Consistent with their localization patterns, we found that these helicases are required for ribosomal RNA processing and regulation of gene expression. Surprisingly, depletion of these helicases resulted in misregulation of highly overlapping sets of protein-coding genes, including many genes that function in differentiation and development. We conclude that R-loop-interacting DEAD-box helicases have non-redundant roles that are critical for maintaining the normal embryonic stem cell transcriptome.

2021-09-17 | PXD022697 | Pride

Perilipin 2 mediates milk fat globule secretion

Project description:Analysis of the milk fat globule membrane proteome from mice with genetic deletion of Perilipin 2 (Plin2/ADRP/ADPH)

2022-10-13 | PXD035020 | Pride

Potential Functions of Histone H3.3K56 Acetylation in Mammals

Project description:To decipher the functions of histone H3.3 acetylation at lysine 56 in mammals using a cellular system.

2022-06-09 | PXD022580 | Pride

Urinary polypeptide ETD/CID analysis, part 2

Project description:Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and protein degradation products, which have been filtered from blood plasma, thus urine has strong potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, to demonstrate that peptides are readily observed. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.

2016-02-15 | PXD002346 | Pride

Urinary polypeptide ETD/CID analysis, part 3

Project description:Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and polypeptidic protein degradation products, which have been filtered from blood plasma, thus urine has potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, and demonstrate ready observation of large polypeptide. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.

2016-02-15 | PXD002372 | Pride

Urinary polypeptide ETD/CID analysis, part 1

2016-02-15 | PXD002312 | Pride

Alpha-1-antitrypsin binds to the glucocorticoid receptor with anti-inflammatory and anti-mycobacterial significance in macrophages

Project description:Alpha-1-antitrypsin (AAT), a serine protease inhibitor produced mainly by the liver, is the third most abundant protein in plasma. Individuals who possess homozygous Z-AAT genotype have severe AAT deficiency and are at risk for early-onset emphysema, bronchiectasis, cirrhosis, panniculitis, and vasculitis. While a canonical receptor for AAT has not been identified, AAT can be internalized into the cytoplasm and is known to affect gene regulation. Since AAT has significant anti-inflammatory properties affecting many cell types including macrophages, we examined whether AAT binds the cytoplasmic glucocorticoid receptor (GR) in macrophages. We report the novel finding that AAT binds to GR in macrophages using several approaches, including co-immunoprecipitation, mass spectrometry, microscale thermophoresis, and in silico molecular modeling. We further demonstrate that AAT induction of angiopoietin-like 4 protein and AAT inhibition of lipopolysaccharide-induced nuclear factor-kappa B activation and interleukin-8 production are mediated, in part, through AAT–GR interaction. Furthermore, this interaction contributes to a host-protective role against Mycobacterium tuberculosis in macrophages. The interaction of AAT and GR described in this study identifies a mechanism for the anti-inflammatory and host-protective properties of AAT.

2022-08-24 | PXD030989 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data