Phosphorylation of S.pombe Rap1 analysed by LC-MS/MS

ABSTRACT: Identification and semi-quantitation of phosphorylation sites on Schizosaccharomyces pombe DNA-binding protein Rap1. A Rap1 construct was cultured in homozygous diploid temperature-sensitive pat1-114 mutant strain of S. pombe cells (time points: 3.5 h and 4.5h), and was purified by anti-PK immunoprecipitation. The resulting samples were run into a 1D SDS-PAGE gel with the target band excised, tryptically digested and analysed by LC-MS/MS using a Velos Orbitrap mass spectrometer.

INSTRUMENT(S):

ORGANISM(S): Schizosaccharomyces Pombe

TISSUE(S): Cell Culture, Diploid Cell

DISEASE(S): Disease Free

SUBMITTER: Andrew Thompson  

LAB HEAD: Andrew James Thompson

PROVIDER: PXD001841 | Pride | 2016-04-27

REPOSITORIES: Pride