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Yeast inter-species comparative proteomics

ABSTRACT: Different yeast species in distinct clades within Saccharomycetaceae family are interesting model organisms for inter-species comparative proteomics, in regard to different metabolic properties and genomic architectures. The aim in this analysis is revealing conserved and diverse expression profile of proteome under different culture conditions, including fermentative and non-fermentative growth. Our label-free quantitative proteomics analysis not only uncovered species-specific characters of metabolic proteins expression pattern and also provide intriguing insight into optimized protein cost allocation and evolutionary role for duplicated genes.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Saccharomyces Mikatae Ifo 1815

TISSUE(S): Cell Culture

SUBMITTER: Keiji Kito

LAB HEAD: Keiji Kito

PROVIDER: PXD001866 | Pride | 2015-11-16

REPOSITORIES: Pride

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Yeast Interspecies Comparative Proteomics Reveals Divergence in Expression Profiles and Provides Insights into Proteome Resource Allocation and Evolutionary Roles of Gene Duplication.

Kito Keiji K Ito Haruka H Nohara Takehiro T Ohnishi Mihoko M Ishibashi Yuko Y Takeda Daisuke D

Molecular & cellular proteomics : MCP 20151111 1

Omics analysis is a versatile approach for understanding the conservation and diversity of molecular systems across multiple taxa. In this study, we compared the proteome expression profiles of four yeast species (Saccharomyces cerevisiae, Saccharomyces mikatae, Kluyveromyces waltii, and Kluyveromyces lactis) grown on glucose- or glycerol-containing media. Conserved expression changes across all species were observed only for a small proportion of all proteins differentially expressed between th ...[more]

PMID: 26560065

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