Proteomics

Dataset Information

Arabidopsis endomembrane enrichments: Affinity enrichment of YFP-RABG3f

ABSTRACT: Our aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations of endosomal proteomes associated with endomembrane markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, and RABG3f as markers for combinations of the Golgi, trans-Golgi Network (TGN), Early Endosomes (EE), secretory vesicles, Late Endosomes (LE), Multivesicular Bodies (MVB) compartments and tonoplast. As comparisons we used Golgi transport 1 (GOT1), which localises to the Golgi, Clathrin Light Chain 2 (CLC2) labelling Clathrin-coated vesicles and pits and the Vesicle Associated Membrane Protein 711 (VAMP711) present at the tonoplast. We developed an easy-to-use method by refining published protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker proteins in Arabidopsis thaliana seedlings.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Alex Jones  

LAB HEAD: Alexandra M. E. Jones

PROVIDER: PXD001881 | Pride | 2015-04-22

REPOSITORIES: Pride

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merged_wh120723_W5 (F004714).mzid_merged_wh120723_W5_(F004714).pride.mgf.gz Mzid
merged_wh120723_W5 (F004714).pride.mztab.gz Mztab
merged_wh120723_W5F004714.mzid.gz Mzid
merged_wh120723_W5F004714.mzid_merged_wh120723_W5_F004714.MGF Mzid
merged_wh120809_w5 (F004719).mzid_merged_wh120809_w5_(F004719).pride.mgf.gz Mzid
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