Proteomics

Dataset Information

Arabidopsis endomembrane enrichments: Affinity enrichment of RABF2b/RFP-ARA7

ABSTRACT: Our aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations of endosomal proteomes associated with endomembrane markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, and RABG3f as markers for combinations of the Golgi, trans-Golgi Network (TGN), Early Endosomes (EE), secretory vesicles, Late Endosomes (LE), Multivesicular Bodies (MVB) compartments and tonoplast. As comparisons we used Golgi transport 1 (GOT1), which localises to the Golgi, Clathrin Light Chain 2 (CLC2) labelling Clathrin-coated vesicles and pits and the Vesicle Associated Membrane Protein 711 (VAMP711) present at the tonoplast. We developed an easy-to-use method by refining published protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker proteins in Arabidopsis thaliana seedlings.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Alex Jones  

LAB HEAD: Alexandra M. E. Jones

PROVIDER: PXD001883 | Pride | 2015-04-22

REPOSITORIES: Pride

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merged_wh120425_R7 (F004701).mzid_merged_wh120425_R7_(F004701).pride.mgf.gz Mzid
merged_wh120425_R7 (F004701).pride.mztab.gz Mztab
merged_wh120425_R7F004701.mzid.gz Mzid
merged_wh120425_R7F004701.mzid_merged_wh120425_R7_F004701.MGF Mzid
merged_wh120517_R7 (F004707).mzid_merged_wh120517_R7_(F004707).pride.mgf.gz Mzid
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