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A quantitative model for macrophage activation predicts tunable distance thresholds for propagation of inflammationes in response LPS stimulation

ABSTRACT: Label free analysis of the secretome of RAW264.7 murine macrophages in response to LPS stimulation.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Laboratory Infectious Disease

SUBMITTER: william rowe

LAB HEAD: Pawel Paszek

PROVIDER: PXD001905 | Pride | 2015-11-30

REPOSITORIES: Pride

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Quantitative analysis of competitive cytokine signaling predicts tissue thresholds for the propagation of macrophage activation.

Bagnall James J Boddington Christopher C England Hazel H Brignall Ruth R Downton Polly P Alsoufi Zainab Z Boyd James J Rowe William W Bennett Alexander A Walker Catherine C Adamson Antony A Patel Nisha M X NMX O'Cualain Ronan R Schmidt Lorraine L Spiller David G DG Jackson Dean A DA Müller Werner W Muldoon Mark M White Michael R H MRH Paszek Pawel P

Science signaling 20180724 540

Toll-like receptor (TLR) signaling regulates macrophage activation and effector cytokine propagation in the constrained environment of a tissue. In macrophage populations, TLR4 stimulates the dose-dependent transcription of nuclear factor κB (NF-κB) target genes. However, using single-RNA counting, we found that individual cells exhibited a wide range (three orders of magnitude) of expression of the gene encoding the proinflammatory cytokine tumor necrosis factor-α (TNF-α). The TLR4-induced <i>T ...[more]

PMID: 30042130

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