Proteomics

Dataset Information

0

SuperQuant: a Data Processing Approach to Increase Quantitative Proteome Coverage


ABSTRACT: SuperQuant is a quantitative proteomics data processing workflow that utilizes fragment ion complementarity to identify multiple co-isolated peptides in tandem mass spectra. Parent ion and label-free quantification method are supported. The performance of the developed approach was tested using dimethyl labeled HeLa lysate sample having artificially created ratio between channels (10(heavy):4(medium):1(light)). Developed software is implemented as a processing node to Thermo Proteome Discoverer 2.x and freely available for the community at https://github.com/caetera/SuperQuantNode.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell Of Cervix, Hela Cell

DISEASE(S): Disease Free

SUBMITTER: Vladimir Gorshkov  

LAB HEAD: Frank Kjeldsen

PROVIDER: PXD001907 | Pride | 2015-05-26

REPOSITORIES: Pride

altmetric image

Publications

SuperQuant: A Data Processing Approach to Increase Quantitative Proteome Coverage.

Gorshkov Vladimir V   Verano-Braga Thiago T   Kjeldsen Frank F  

Analytical chemistry 20150603 12


SuperQuant is a quantitative proteomics data processing approach that uses complementary fragment ions to identify multiple coisolated peptides in tandem mass spectra allowing for their quantification. This approach can be applied to any shotgun proteomics data set acquired with high mass accuracy for quantification at the MS(1) level. The SuperQuant approach was developed and implemented as a processing node within the Thermo Proteome Discoverer 2.x. The performance of the developed approach wa  ...[more]

Similar Datasets

2018-03-08 | PXD005245 | Pride
2011-07-06 | E-GEOD-30404 | biostudies-arrayexpress
2011-02-08 | E-GEOD-25687 | biostudies-arrayexpress
2011-08-22 | E-GEOD-27604 | biostudies-arrayexpress
2011-02-08 | E-GEOD-21720 | biostudies-arrayexpress
2016-02-28 | E-MTAB-3247 | biostudies-arrayexpress
2019-07-16 | PXD013083 | Pride
2021-03-18 | PXD012084 | Pride
2015-05-12 | PXD001039 | Pride
2022-05-11 | PXD029754 | Pride