AP-MS of GFP-MCM2 in the cellular response to etoposide induced DNA damage.

ABSTRACT: SILAC quantitative proteomics using immunoprecipitation of GFP-tagged fusion proteins to identify proteins interacting with the MCM complex, and quantify changes in interactions in response to DNA damage. U2OS cells expressing GFP-MCM2 were used to purify the MCM complex following treatment or not with etoposide, a topoisomerase II inhibitor causing DNA damage.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Osteosarcoma

SUBMITTER: Francois-Michel Boisvert  

LAB HEAD: Francois-Michel Boisvert

PROVIDER: PXD001949 | Pride | 2017-12-06

REPOSITORIES: Pride