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Dynamic interaction- and phosphoproteomics of CD147

ABSTRACT: Using affinity purification, mass spectrometry, and phospho-enrichment of isotope-labeled peptides, we examined the CD147 microenvironment and CD147-dependent signaling cascades in the Jurkat acute T cell leukemic cell line and its dynamics upon treatment with T cell stimulating agents.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Nagarjuna Nagaraj

LAB HEAD: Hannes Stockinger

PROVIDER: PXD002055 | Pride | 2017-02-06

REPOSITORIES: Pride

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Dynamic Interaction- and Phospho-Proteomics Reveal Lck as a Major Signaling Hub of CD147 in T Cells.

Supper Verena V Hartl Ingrid I Boulègue Cyril C Ohradanova-Repic Anna A Stockinger Hannes H

Journal of immunology (Baltimore, Md. : 1950) 20170201 6

Numerous publications have addressed CD147 as a tumor marker and regulator of cytoskeleton, cell growth, stress response, or immune cell function; however, the molecular functionality of CD147 remains incompletely understood. Using affinity purification, mass spectrometry, and phosphopeptide enrichment of isotope-labeled peptides, we examined the dynamic of the CD147 microenvironment and the CD147-dependent phosphoproteome in the Jurkat T cell line upon treatment with T cell stimulating agents. ...[more]

PMID: 28148733

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