Wheat Allergens MALDI-TOF/TOF - Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts

ABSTRACT: The aim of the work was to test relatively simple proteomics approach based on phenol extraction and two-dimensional gel electrophoresis (2-DE) with 7cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain. Using this approach, 157 2-DE spots were quantified in biological triplicate, out of which 55 were identified by MALDI-TOF/TOF mass spectrometry. Clinically relevant proteins associated with celiac disease, wheat dependent exercise induced anaphylaxis, baker´s asthma, and food allergy, were detected in 24 2-DE spots. However, alcohol-soluble gliadins were not detected with this approach. The comparison with recent quantitative study suggested that gel-based and gel-free proteomics approaches are complementary for the detection and quantification of clinically relevant proteins in wheat grain.

INSTRUMENT(S): ultraflex

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Plant Cell, Endosperm

DISEASE(S): Hypersensitivity Reaction Type I Disease

SUBMITTER: Maksym Danchenko  

LAB HEAD: Martin Hajduch

PROVIDER: PXD002067 | Pride | 2017-08-23

REPOSITORIES: Pride