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Comprehensive protein interactome analysis of a key RNA helicase: the detection of novel stress granule proteins

ABSTRACT: We conducted an unbiased large-scale study to map the protein-protein interactome of DDX6 using immunoprecipitation and mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Nerea Cuevas Polo

LAB HEAD: Christine Vogel

PROVIDER: PXD002070 | Pride | 2015-07-27

REPOSITORIES: Pride

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			Action	DRS
	130122_293.raw	Raw
	130122_293_benzonase.raw	Raw
	130122_DDX6.raw	Raw
	130122_DDX6_benzonase.raw	Raw
	130125_293.raw	Raw

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Publications

Comprehensive Protein Interactome Analysis of a Key RNA Helicase: Detection of Novel Stress Granule Proteins.

Bish Rebecca R Cuevas-Polo Nerea N Cheng Zhe Z Hambardzumyan Dolores D Munschauer Mathias M Landthaler Markus M Vogel Christine C

Biomolecules 20150715 3

DDX6 (p54/RCK) is a human RNA helicase with central roles in mRNA decay and translation repression. To help our understanding of how DDX6 performs these multiple functions, we conducted the first unbiased, large-scale study to map the DDX6-centric protein-protein interactome using immunoprecipitation and mass spectrometry. Using DDX6 as bait, we identify a high-confidence and high-quality set of protein interaction partners which are enriched for functions in RNA metabolism and ribosomal protein ...[more]

PMID: 26184334

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