Proteomics

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Proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici


ABSTRACT: Z. tritici is a fungal pathogen causing the disease septoria tritici blotch, one of the most economically devastating foliar diseases in wheat. The molecular basis underlying Z. tritici growth, development and pathogenicity is not fully understood yet. Compared to the genomic investigations in this fungus, little is known about the protein expression at a systematic level. The aim of the project is to construct a comprehensive protein database of Z. tritici growing in nutrient-limiting and rich media and in vivo at a late stage of wheat infection by using 1D gel-based and SCX-based proteomics and subproteomics (intracellular and extracellular) approaches.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zymoseptoria Tritici (strain Cbs 115943 / Ipo323) (speckled Leaf Blotch Fungus) (septoria Tritici)

TISSUE(S): Cell Culture, Leaf

SUBMITTER: Fen Yang  

LAB HEAD: Fen Yang

PROVIDER: PXD002147 | Pride | 2018-07-05

REPOSITORIES: Pride

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Publications

Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici.

Yang Fen F   Yin Qi Q  

Proteomics 20151124 1


Zymoseptoria tritici causes Septoria tritici blotch disease of wheat. To obtain a comprehensive protein dataset of this fungal pathogen, proteomes of Z. tritici growing in nutrient-limiting and rich media and in vivo at a late stage of wheat infection were fractionated by 1D gel or strong cation exchange (SCX) chromatography and analyzed by LC-MS/MS. A total of 5731, 5376 and 3168 Z. tritici proteins were confidently identified from these conditions, respectively. Of these in vitro and in planta  ...[more]

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