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Quantitative analysis of temporal IP and cell lysate using SWATH-MS (1)

ABSTRACT: We treated L929 cells with TNF for six time points and the samples containing TNFR1 complexes were collected by affinity purification and analyzed by SWATH-MS. Accurate and consistent quantification across 18 IP samples showed dynamic profiles of the TNFR1 complex components across six time points.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Chuan-Qi Zhong

LAB HEAD: Chuan-Qi Zhong

PROVIDER: PXD002177 | Pride | 2021-01-11

REPOSITORIES: Pride

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Group-DIA: analyzing multiple data-independent acquisition mass spectrometry data files.

Li Yuanyue Y Zhong Chuan-Qi CQ Xu Xiaozheng X Cai Shaowei S Wu Xiurong X Zhang Yingying Y Chen Jinan J Shi Jianghong J Lin Shengcai S Han Jiahuai J

Nature methods 20151201 12

PMID: 26436481

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Quantitative analysis of temporal IP and cell lysate using SWATH-MS (2)

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