Proteomics

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CN-CHPP 2015 Testis Project - Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins

ABSTRACT: The testis tissue-based trans-omics was a feasible approach to find more missing proteins (MPs) because it was reported higher gene expression found in testis tissues. Three testis tissues and two protein separation strategies were selected to quantitatively evaluate gene expression abundance at the proteomic and transcriptomic levels. The project have been jointly supervised by Ping Xu and Siqi Liu.

REANALYSED by: PAe005356PAe005333

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Testis

SUBMITTER: Bo Wen  

LAB HEAD: Siqi Liu

PROVIDER: PXD002179 | Pride | 2015-11-03

REPOSITORIES: Pride

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Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics of testis tissue was a feasible approach to identify more MPs because testis tissues have higher gene expression levels. Here we combined proteomics and transcriptomics to survey gene expression in human testis tissues from three post-mortem individuals. Proteins were extracted and separated with glycine- and tricine-SDS-PAGE. A total of 9597 protein groups were identifie  ...[more]