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Fly Stress - Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster

ABSTRACT: Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. While ageing and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this we used a redox proteomic technique, Oxidative Isotope Coded Affinity TAgs (OxICAT), to assess cysteine residue redox changes in Drosophila melanogaster during ageing and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Whole Body

SUBMITTER: Andrew James

LAB HEAD: Michael Patrick Murphy

PROVIDER: PXD002195 | Pride | 2018-10-24

REPOSITORIES: Pride

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Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster.

Menger Katja E KE James Andrew M AM Cochemé Helena M HM Harbour Michael E ME Chouchani Edward T ET Ding Shujing S Fearnley Ian M IM Partridge Linda L Murphy Michael P MP

Cell reports 20150618 12

Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT), to assess cysteine-residue redox changes in Drosophila melanogaster during aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of s ...[more]

PMID: 26095360

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