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An effective plasma membrane proteomics approach for small tissue samples

ABSTRACT: Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of ? 1600 proteins of which > 60% are known to be associated to the plasma membrane according to GO annotations, including (G-protein coupled) receptors, ion channels and transporters, and this from mm3-scale tissue.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Dirk Valkenborg

LAB HEAD: Dirk Valkenborg

PROVIDER: PXD002227 | Pride | 2022-02-22

REPOSITORIES: Pride

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An effective plasma membrane proteomics approach for small tissue samples.

Smolders Katrien K Lombaert Nathalie N Valkenborg Dirk D Baggerman Geert G Arckens Lutgarde L

Scientific reports 20150605

Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm(3)-scale ...[more]

PMID: 26047021

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