Browse
Submit Data
Databases
API
Help

Dataset Information

457 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome and pathway effects of chronic haoperidol treatment in mouse hippocampus

ABSTRACT: Seven male C57BL/6 mice, at 10 weeks of age, were injected daily intraperitoneally with 0.5 mg/kg of haloperidol, for 28 days. A control group of six animals was injected with vehicle only (saline).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Melanie Foecking

LAB HEAD: David Richard Cotter

PROVIDER: PXD002250 | Pride | 2015-11-30

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Proteome and pathway effects of chronic haloperidol treatment in mouse hippocampus.

Schubert Klaus Oliver KO Föcking Melanie M Wynne Kieran K Cotter David R DR

Proteomics 20160113 3

Proteomic exploration of the effects of psychotropic drugs on specific brain areas in rodents has the potential to uncover novel molecular networks and pathways affected by psychotropic medications, and may inform etiologic hypotheses on mental disorders. Haloperidol, a widely used first-generation antipsychotic, has been shown to produce structural and functional changes of the hippocampus, a brain region also implicated in the neuropathology of disorders such as schizophrenia and bipolar disor ...[more]

PMID: 26607048

Similar Datasets

TMT quantitative proteomics of a) protrusions and cells bodies of a panel of cell lines, b) protrusions and cells bodies of MDA-MB231 cells collected after 1 h, 2 h, 4 h, 8 h post- induction of protrusions, c) non-transfected (nt) or LARP6 siRNAs MDA-MB231 cells grown on either close or open collagen coated transwells and d) protrusions and cells bodies of a panel of breast cell lines.

Project description:Translation of Ribosomal Protein coding mRNAs (RP-mRNAs) constitutes a key step in regulation of ribosome biogenesis in human cells, but the exact mechanisms which modulate RP-mRNAs translation under various cellular and environmental conditions remain poorly understood. Here we show that the subcellular localisation of RP-mRNAs acts as a key regulator of their translation in mesenchymal-like migratory cells. As cells invade into their surroundings, RP-mRNAs localise to the actin-rich protrusions at the front of the cells. This localisation is mediated by La related protein-6 (LARP6), an RNA Binding Protein (RBP) that is enriched in protrusions. Importantly, translation initiation and elongation factors are also enriched in protrusions. LARP6 dependent localisation of RP-mRNAs enhances their translation, leading to up-regulation of ribosome biogenesis and increased overall protein synthesis. In breast carcinomas, enhanced expression of LARP6 is associated with Epithelial to Mesenchymal Transition (EMT), and can be therapeutically targeted by a small molecule inhibitor which interferes with LARP6 RNA binding. These findings reveal an RNA localisation based post-transcriptional mechanism that governs ribosome biogenesis in migratory cells, and implicate a role for this process in cancer progression downstream of EMT.

2021-09-08 | PXD015808 | Pride

O-GlcNAcylation of RIPK1 Rescues Red Blood Cells from Necroptosis

Project description:Necroptosis is a type of cell death with excessive inflammation and organ damage in various human diseases. Although abnormal necroptosis is common in patients with neurodegenerative, cardiovascular, and infectious diseases, the mechanisms by which O-GlcNAcylation contributes to the regulation of necroptotic cell death are poorly understood. In this study, we reveal that O- GlcNAcylation of RIPK1 (receptor-interacting protein kinase1) was decreased in erythrocytes of the mouse injected with lipopolysaccharide, resulting in the acceleration of erythrocyte necroptosis through increased formation of RIPK1-RIPK3 complex. Mechanistically, we discovered that O- GlcNAcylation of RIPK1 at serine 331 in human (corresponding to serine 332 in mouse) inhibits 32 This is a provisional file, not the final typeset article phosphorylation of RIPK1 at serine 166, which is necessary for the necroptotic activity of RIPK1 and suppresses the formation of the RIPK1-RIPK3 complex in Ripk1 -/- MEFs. Thus, our study demonstrates that RIPK1 O-GlcNAcylation serves as a checkpoint to suppress necroptotic signaling in erythrocytes.

2023-06-06 | PXD042579 | Pride

Proteomic and morphological effect of nanosilver on soybean under flooding stress

Project description:Flooding stress has a negative impact on the soybean cultivation in early growth stages. In order to understand the effect of nanosilver on the soybean growth under flooding stress, quantitative proteomics technique was used.

2016-12-14 | PXD001579 | Pride

Insights into the proteomic response of soybean to stress of Al2O3, ZnO, and Ag nanoparticles

Project description:The present study highlights the phytotoxicity of nanoparticles (NPs) to soybean roots and leaves at proteome level. The effect of three NPs Al2O3, ZnO, and Ag suspensions on soybean was evaluated.

2015-11-16 | PXD001923 | Pride

Proteomic analysis of the effect of aluminum oxide nanoparticles on soybean root under flooding stress

Project description:Flooding stress has a negative impact on the soybean cultivation in early growth stages. In order to understand the effect of aluminum oxide nanoparticles on the soybean growth under flooding stress, quantitative proteomics technique was used.

2015-08-31 | PXD002005 | Pride

Quantitative proteomics of soybean root during recovery after flooding

Project description:Soybean is one of the most economically important legume in the world, providing vegetable protein and oil for millions of people, ingredients for hundreds of nutritional products, but its production being threatened globally by increased flooding events. To unravel the mechanism involved in recovery after flooding in soybean, proteomic analysis was carried out.

2014-12-04 | PXD000986 | Pride

Integrated seed proteome and phosphoproteome analyses reveal interplay of nutrient dynamics, carbon-nitrogen partitioning and oxidative signaling in chickpea, part 3

Project description:To understand nutrient dynamics during embryonic and cotyledonary photoheterotrophic transition to mature and germinating autotrophic seeds, TiO2-based phosphoproteomics study in three sequential seed developmental phases in chickpea was performed.

2020-03-11 | PXD017516 | Pride

LC-MS/MS Data for Identification of ChREBP O-GlcNAcylation

Project description:LC-MS/MS analysis of O-GlcNAcylated ChREBP proteins, which were overexpressed in HEK293T cells and purified via affinity purification using Ni-NTA agarose

2017-05-04 | PXD004286 | Pride

Neocortical and hippocampal gene expression in kainate- and nicotine-injected juvenile mice

Project description:To examine irreversible changes in the developing brain following seizures, juvenile inbred mice were intraperitoneally injected with kainate and nicotine. Keywords: seizure induction

2007-01-04 | GSE6388 | GEO

Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress

Project description:Flooding stress has a negative impact on soybean cultivation because it severely impairs growth and development. To understand the flooding responsive mechanism in early stage soybeans, a glycoproteomic technique was used

2014-12-08 | PXD001350 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data