Identification and characterization of DNA sequences that restrict genomic binding of the glucocorticoid receptor

ABSTRACT: The glucocorticoid receptor (GR), is a hormone-activated transcription factor which binds to GR binding sequences (GBS) encoded in the genome. Interestingly, of the many genomic sequences matching the GR consensus sequence only a small subset is actually bound. This indicates that the presence of a GBS alone is insufficient to specify where in the genome GR binds. We identified sequences that can locally prevent GR binding and mass-spec analysis was used to identify proteins that bind to these sequences.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: David Meierhofer  

LAB HEAD: Sebastiaan H. Meijsing

PROVIDER: PXD002252 | Pride | 2016-03-30

REPOSITORIES: Pride