Proteomics

Dataset Information

94

Identification and characterization of DNA sequences that restrict genomic binding of the glucocorticoid receptor


ABSTRACT: The glucocorticoid receptor (GR), is a hormone-activated transcription factor which binds to GR binding sequences (GBS) encoded in the genome. Interestingly, of the many genomic sequences matching the GR consensus sequence only a small subset is actually bound. This indicates that the presence of a GBS alone is insufficient to specify where in the genome GR binds. We identified sequences that can locally prevent GR binding and mass-spec analysis was used to identify proteins that bind to these sequences.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo sapiens  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: David Meierhofer  

LAB HEAD: Sebastiaan H. Meijsing

PROVIDER: PXD002252 | Pride | 2016-03-30

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
140113_Jonas_U1.RAW Raw
140113_Jonas_U2.RAW Raw
140113_Jonas_U3.RAW Raw
140113_Jonas_neg.RAW Raw
220213_Jonas_Control_1EI.RAW Raw
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Publications

Identification and characterization of DNA sequences that prevent glucocorticoid receptor binding to nearby response elements.

Telorac Jonas J   Prykhozhij Sergey V SV   Schöne Stefanie S   Meierhofer David D   Sauer Sascha S   Thomas-Chollier Morgane M   Meijsing Sebastiaan H SH  

Nucleic acids research 20160325 13


Out of the myriad of potential DNA binding sites of the glucocorticoid receptor (GR) found in the human genome, only a cell-type specific minority is actually bound, indicating that the presence of a recognition sequence alone is insufficient to specify where GR binds. Cooperative interactions with other transcription factors (TFs) are known to contribute to binding specificity. Here, we reasoned that sequence signals preventing GR recruitment to certain loci provide an alternative means to conf  ...[more]

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