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High Fat Diet Related Changes in Mitochondrial Proteome

ABSTRACT: Western diets leads to obesity and its comorbidities, in particular the progression of hepatosteatosis and insulin resistance. In DIO C57/BL6 obese mice, we performed quantitative mitochondrial bioenergetics and proteomic analysis and fused both technologies to understand the functional adjustments of mitochondrial energy metabolism in response to overfeeding.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Jacek Wisniewski

LAB HEAD: Jacek R. Wisniewski

PROVIDER: PXD002289 | Pride | 2018-02-20

REPOSITORIES: Pride

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Respiromics - An integrative analysis linking mitochondrial bioenergetics to molecular signatures.

Walheim Ellen E Wiśniewski Jacek R JR Jastroch Martin M

Molecular metabolism 20180105

<h4>Objective</h4>Energy metabolism is challenged upon nutrient stress, eventually leading to a variety of metabolic diseases that represent a major global health burden.<h4>Methods</h4>Here, we combine quantitative mitochondrial respirometry (Seahorse technology) and proteomics (LC-MS/MS-based total protein approach) to understand how molecular changes translate to changes in mitochondrial energy transduction during diet-induced obesity (DIO) in the liver.<h4>Results</h4>The integrative analysi ...[more]

PMID: 29361498

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