Dataset Information

Proteome Profiling in Lung Injury Following Hematopoietic Stem Cell Transplantation

ABSTRACT: The goal of this study was to apply protein expression profiling tools in cases with lung injury following HSCT or cellular therapy infusion to identify the proteins and biological processes that differentiate IPS from infectious lung injury in HSCT recipients. We performed comprehensive label-based quantitative protein profiling of BALF in patients undergoing HSCT

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bronchoalveolar Lavage

DISEASE(S): Adult Respiratory Distress Syndrome

SUBMITTER: Kevin Viken

LAB HEAD: Maneesh Bhargava

PROVIDER: PXD002437 | Pride | 2018-10-24

REPOSITORIES: Pride

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Publications

Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation.

Bhargava Maneesh M Viken Kevin J KJ Dey Sanjoy S Steinbach Michael S MS Wu Baolin B Jagtap Pratik D PD Higgins LeeAnn L Panoskaltsis-Mortari Angela A Weisdorf Daniel J DJ Kumar Vipin V Arora Mukta M Bitterman Peter B PB Ingbar David H DH Wendt Chris H CH

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 20160505 8

Pulmonary complications due to infection and idiopathic pneumonia syndrome (IPS), a noninfectious lung injury in hematopoietic stem cell transplant (HSCT) recipients, are frequent causes of transplantation-related mortality and morbidity. Our objective was to characterize the global bronchoalveolar lavage fluid (BALF) protein expression of IPS to identify proteins and pathways that differentiate IPS from infectious lung injury after HSCT. We studied 30 BALF samples from patients who developed lu ...[more]

PMID: 27155584

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Project description:The goal of this study was to investigate the effects of magnetic iron cobalt oxide nanoparticles (cobalt doped Fe3O4 nanoparticles with increasing amounts of cobalt) after pulmonary exposure while in parallel presenting a proteomics platform that is easily transferable to large-scale nanotoxicology screening as part of an integrated assessment and testing approach for regulation of nanoparticles. Bronchoalveolar lavage fluid (BALF) is a proximal biofluid that can be used to monitor airway inflammation and toxic responses in the lung. It is routinely sampled for differential lung diagnostics and has been discussed as a source for early detection of lung cancer. In order to assess effects of metal oxide nanoparticles upon inhalation, bronchoalveolar lavage fluid from mice dosed by single intratracheal instillation was collected and subjected to classical biocompatibility assays as well as proteome analysis. Magnetic oxide nanoparticles with iron and cobalt oxide (Fe3-xCoxO4) at different ratios (1:0, 3:1, 1:1, 1:3, 0:1) were tested at two concentrations (54 µg, 162 µg per animal) and two time points after instillation (day 1, day 3). As a positive control carbon black nanomaterial known to induce lung inflammation was included for both time points, but only at the high dosage (162 µg per animal). Proteomics experiments were divided into three parts – test of reproducibility, discovery and screening phase. The reproducibility of the newly introduced Evosep One LC system was evaluated by re-measuring of technical replicates (n=16). During the discovery phase, selected representative samples with 3 biological replicates per group (total n=9) including pure iron oxide nanoparticles, pure cobalt oxide nanoparticles and vehicle controls were subjected to in-depth proteome profiling by extensive pre-fractionation and including isobaric tandem mass tag (TMT) labeling following a classical LC-MS/MS setup. This step allowed us to identify affected pathways and generate hypotheses regarding mechanisms of the effects of nanoparticles. During screening, all samples of the study were measured label-free as single-shot injections separated on short gradients of 21 min using the robust, high-throughput Evosep One LC system. This step allowed a fast screening of the 5 different types of magnetic metal oxide nanoparticles on BALF, at two concentrations and two time points together with their representative controls (total n=166). All screening measurements were completed in only 2.7 days.

Dataset Information

Proteome Profiling in Lung Injury Following Hematopoietic Stem Cell Transplantation

Publications

Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation.

Similar Datasets

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