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EMT induction in MCF7 cells by overexpression of SNAIL transcription factor

ABSTRACT: The human breast cancer cell line MCF7 was trasnduced with SNAIL trasncription factor. In-depth proteomic analysis revealed several important cellular processes regulated, especially related to epigenetic control of cell proliferation and protein expression.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Vitor Faca

LAB HEAD: Vitor Faca

PROVIDER: PXD002449 | Pride | 2016-01-20

REPOSITORIES: Pride

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Proteomic Analysis of Epithelial to Mesenchymal Transition (EMT) Reveals Cross-talk between SNAIL and HDAC1 Proteins in Breast Cancer Cells.

Palma Camila de Souza Cde S Grassi Mariana Lopes ML Thomé Carolina Hassibe CH Ferreira Germano Aguiar GA Albuquerque Daniele D Pinto Mariana Tomazini MT Ferreira Melo Fernanda Ursoli FU Kashima Simone S Covas Dimas Tadeu DT Pitteri Sharon J SJ Faça Vitor M VM

Molecular & cellular proteomics : MCP 20160113 3

Epithelial to mesenchymal transition (EMT)(1) occurs naturally during embryogenesis, tissue repair, cancer progression, and metastasis. EMT induces cellular and microenvironmental changes resulting in loss of epithelial and acquisition of mesenchymal phenotypes, which promotes cellular invasive and migratory capabilities. EMT can be triggered by extracellular factors, including TGF-β, HGF, and EGF. Overexpression of transcription factors, such as SNAIL, SLUG, ZEB1/2, and TWIST1, also induces EMT ...[more]

PMID: 26764010

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