Proteomics

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Facile Discovery of Cell-surface Protein Targets of Cancer Cell Aptamers


ABSTRACT: Cancer biomarker discovery constitutes a frontier in cancer research. In recent years, cell-binding aptamers have become useful molecular probes for biomarker discovery. However, there are few successful examples, and the critical barrier resides in the identification of the cell-surface protein targets for the aptamers, where only a limited number of aptamer targets have been identified so far. Herein, we developed a universal SILAC-based quantitative proteomic method for target discovery of cell-binding aptamers. The method allowed for distinguishing specific aptamer-binding proteins from non-specific proteins based on abundance ratios of proteins bound to aptamer-carrying bait and control bait. In addition, we employed fluorescently labeled aptamers for monitoring and optimizing the binding conditions. We were able to identify and validate selectin L and integrin 4 as the protein targets for two previously reported aptamers, Sgc-3b and Sgc-4e, respectively. This strategy should be generally applicable for the discovery of protein targets for other cell-binding aptamers, which will promote the applications of these aptamers.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Jurkat Cell

DISEASE(S): Acute Leukemia

SUBMITTER: Tao Bing  

LAB HEAD: Yinsheng Wang

PROVIDER: PXD002480 | Pride | 2022-02-28

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
120-SALIC-A_150124234103.raw Raw
120-SALIC-C.raw Raw
1218-A-2.raw Raw
1218SALIC-D.raw Raw
1231-BHJL.raw Raw
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Publications

Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers.

Bing Tao T   Shangguan Dihua D   Wang Yinsheng Y  

Molecular & cellular proteomics : MCP 20150721 10


Cancer biomarker discovery constitutes a frontier in cancer research. In recent years, cell-binding aptamers have become useful molecular probes for biomarker discovery. However, there are few successful examples, and the critical barrier resides in the identification of the cell-surface protein targets for the aptamers, where only a limited number of aptamer targets have been identified so far. Herein, we developed a universal SILAC-based quantitative proteomic method for target discovery of ce  ...[more]

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