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Age effects in the extracellular matrix (ECM) proteome of the mouse left ventricle

ABSTRACT: Matrix metalloproteinases (MMPs) collectively degrade all extracellular matrix (ECM) proteins. MMP-9 has the strongest link to development of cardiac dysfunction. Aging is associated with increased MMP-9 expression in the left ventricle (LV) and reduced cardiac function. We investigated the effect of MMP-9 deletion on the cardiac ECM in aged mice. Based on label-free mass spectrometry analyses, MMP-9 dependent age-related changes were found in the mouse cardiac ECM proteome, suggesting MMP-9 as a possible therapeutic target for the aging patient

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Susan Weintraub

LAB HEAD: Susan T. Weintraub

PROVIDER: PXD002488 | Pride | 2018-07-05

REPOSITORIES: Pride

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Matrix metalloproteinase-9-dependent mechanisms of reduced contractility and increased stiffness in the aging heart.

Padmanabhan Iyer Rugmani R Chiao Ying Ann YA Flynn Elizabeth R ER Hakala Kevin K Cates Courtney A CA Weintraub Susan T ST de Castro Brás Lisandra E LE

Proteomics. Clinical applications 20151217 1

<h4>Purpose</h4>Matrix metalloproteinases (MMPs) collectively degrade all extracellular matrix (ECM) proteins. Of the MMPs, MMP-9 has the strongest link to the development of cardiac dysfunction. Aging associates with increased MMP-9 expression in the left ventricle (LV) and reduced cardiac function. We investigated the effect of MMP-9 deletion on the cardiac ECM in aged animals.<h4>Experimental design</h4>We used male and female middle-aged (10- to16-month old) and old (20- to 24-month old) wil ...[more]

PMID: 26415707

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