Proteomics

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Cupiennius salei Hyal LC-MSMS - Isolation, N-glycosylations and Function of a Hyaluronidase-Like Enzyme from the Venom of the Spider Cupiennius salei

ABSTRACT: Hyaluronidases are important venom components by acting as spreading factor of neurotoxins. In several studies this spreading effect was tested on vertebrate tissue. However, data about the spreading activity on invertebrates, which are the main prey organisms of spiders, are lacking. In this study, a hyaluronidase-like enzyme was isolated from the venom of the spider Cupiennius salei. The amino acid sequence of the enzyme was solved by cDNA analysis of the venom gland transcriptome and confirmed by protein analytics. Two complex N-linked glycans, which akin honey bee hyaluronidase glycosylations, were identified by tandem mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Cupiennius Salei (american Wandering Spider)

TISSUE(S): Venom Apparatus

SUBMITTER: Christian Trachsel  

LAB HEAD: Lucia Kuhn-Nentwig

PROVIDER: PXD002489 | Pride | 2015-12-10

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20131203_02_Hyal_trp_digst.msf Msf
20131203_02_Hyal_trp_digst.raw Raw
20131209_02_Hyal_trp_digst.msf Msf
20131209_02_Hyal_trp_digst.raw Raw
20131212_03_Hyal_AspN_digst_1.msf Msf
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Publications

Isolation, N-glycosylations and Function of a Hyaluronidase-Like Enzyme from the Venom of the Spider Cupiennius salei.

Biner Olivier O   Trachsel Christian C   Moser Aline A   Kopp Lukas L   Langenegger Nicolas N   Kämpfer Urs U   von Ballmoos Christoph C   Nentwig Wolfgang W   Schürch Stefan S   Schaller Johann J   Kuhn-Nentwig Lucia L  

PloS one 20151202 12

<h4>Structure of cupiennius salei venom hyaluronidase</h4>Hyaluronidases are important venom components acting as spreading factor of toxic compounds. In several studies this spreading effect was tested on vertebrate tissue. However, data about the spreading activity on invertebrates, the main prey organisms of spiders, are lacking. Here, a hyaluronidase-like enzyme was isolated from the venom of the spider Cupiennius salei. The amino acid sequence of the enzyme was determined by cDNA analysis o  ...[more]

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