Proteomics

Dataset Information

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Identification of GLI-associated proteins by mass spectrometry


ABSTRACT: The Hedgehog signaling pathway is essential for the maintenance and response of several types of stem cells. To study the transcriptional response of stem cells to HH signaling, we searched for proteins binding to GLI proteins, the transcriptional effectors of the HH pathway in mouse embryonic stem (ES) cells. We purified GLI protein complex from an ES cell line that contained a tamoxifen-inducible 3XFLAG-tagged GLI3 repressor allele by anti-FLAG immunoprecipitation and several novel GLI co-factors were identified in the complex by subsequent mass spectrometry analysis.

INSTRUMENT(S): LTQ Orbitrap, LTQ

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

DISEASE(S): Glioblastoma

SUBMITTER: Maria Person  

LAB HEAD: Steven A. Vokes

PROVIDER: PXD002494 | Pride | 2016-02-04

REPOSITORIES: Pride

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Publications

The Pluripotency Factor NANOG Binds to GLI Proteins and Represses Hedgehog-mediated Transcription.

Li Qiang Q   Lex Rachel K RK   Chung HaeWon H   Giovanetti Simone M SM   Ji Zhicheng Z   Ji Hongkai H   Person Maria D MD   Kim Jonghwan J   Vokes Steven A SA  

The Journal of biological chemistry 20160121 13


The Hedgehog (HH) signaling pathway is essential for the maintenance and response of several types of stem cells. To study the transcriptional response of stem cells to HH signaling, we searched for proteins binding to GLI proteins, the transcriptional effectors of the HH pathway in mouse embryonic stem (ES) cells. We found that both GLI3 and GLI1 bind to the pluripotency factor NANOG. The ectopic expression of NANOG inhibits GLI1-mediated transcriptional responses in a dose-dependent fashion. I  ...[more]

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