Dataset Information

Selective phospohorylation of macrophage differentiation

ABSTRACT: The differentiation of macrophages from monocytes is a tightly controlled and complex biological process. Although numerous studies have been conducted using biochemical approaches or global gene/protein profiling, the mechanisms of the early stages of differentiation remain unclear. Here we used THP1 cells to model the differentiation and studied their phosphoproteome perturbation during the early differentiation process

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Huoming Zhang

LAB HEAD: Huoming Zhang

PROVIDER: PXD002496 | Pride | 2015-09-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20110801_THP1_0_30_60min_PhosphoSTYSites.txt	Txt
	20110801_THP1_0_30_60min_a01.raw	Raw
	20110801_THP1_0_30_60min_a02.raw	Raw
	20110801_THP1_0_30_60min_a03.raw	Raw
	20110801_THP1_0_30_60min_a04.raw	Raw

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Publications

Selective phosphorylation during early macrophage differentiation.

Zhang Huoming H Qian Pei-Yuan PY Ravasi Timothy T

Proteomics 20150915 21

The differentiation of macrophages from monocytes is a tightly controlled and complex biological process. Although numerous studies have been conducted using biochemical approaches or global gene/protein profiling, the mechanisms of the early stages of differentiation remain unclear. Here we used SILAC-based quantitative proteomics approach to perform temporal phosphoproteome profiling of early macrophage differentiation. We identified a large set of phosphoproteins and grouped them as PMA-regul ...[more]

PMID: 26307563

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