A method to characterize antibody selectivity immunoprecipitation, part 2

ABSTRACT: Antibodies are used in multiple cell biology applications but there are methods to assess antibody quality, riskingdata integrity and repr developed a standard operating procedure using HEK293 cells to score and antibodies for suitability in immunoprecipitation. This method, validated in in fourindependent laboratories, was developed using 1124 novel recomb for152 chromatin-related human proteins. We used mass spectrometry abundanceof all the proteinsin eachimmunoprecipitateand compared spectral abundance factors from the target antigen with those of all Antibodies for which the targetantigen or a member of its known protein most abundant protein were classified as “IP gold standard”. This m quantitative outputs that can be stored and archived in public databases, step towards a platform for community benchmarking of antibody quality.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Hongbo Guo  

LAB HEAD: Jack Greenblatt

PROVIDER: PXD002534 | Pride | 2015-07-15

REPOSITORIES: Pride