Middle-down MS analysis of intact and clipped histone H3 proteoforms

ABSTRACT: There is growing evidence that proteolytic cleavage of histone N-terminal tails, known as histone clipping, influences nucleosome dynamics and functional properties. Using middle-down MS analysis we characterized intact and proteolytically processed histone H3 proteoforms isolated from human hepatocarcinoma cell line HepG2/C3A grown in 3D culture.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Andrey Tvardovskiy  

LAB HEAD: Ole Nørregaard Jensen

PROVIDER: PXD002535 | Pride | 2022-03-14

REPOSITORIES: Pride