Proteomics

Dataset Information

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Proteomic study of fruit exocarp tissue in four cultivars of Vitis Vinifera


ABSTRACT: The aim of this work was to study the metabolism of grape berry skin, a tissue that has a protective role against damage by physical injuries and pathogen attacks. This role requires a metabolism able to sustain biosynthetic activities such as those relating to secondary compounds (i.e. flavonoids). In order to draw the attention on these biochemical processes, a proteomic and metabolomic comparative analysis was performed among Riesling Italico, Pinot Gris, Pinot Noir and Croatina cultivars, which are known to accumulate anthocyanins to a different extent. The application of multivariate statistics on the dataset pointed out that the cultivars were distinguishable from each other and the order in which they were grouped mainly reflected their relative anthocyanin contents. Sorting the spots according to their significance selected proteins were characterized by LC-ESI-MS/MS. Considering the functional distribution, the identified proteins were involved in many physiological processes such as stress, defense, carbon metabolism, energy conversion and secondary metabolism. The trends of some metabolites were related to those of the protein data. Taken together, the results permitted to highlight the relationships between the secondary compound pathways and the main metabolism (e.g. glycolysis and TCA cycle). Moreover, the trend of accumulation of many proteins involved in stress responses, reinforced the idea that they could play a role in the cultivar specific developmental plan.

INSTRUMENT(S): LCQ Classic

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Exocarp

SUBMITTER: Luca Espen  

LAB HEAD: Luca Espen

PROVIDER: PXD002539 | Pride | 2015-07-27

REPOSITORIES: Pride

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Publications

Proteomic and metabolic traits of grape exocarp to explain different anthocyanin concentrations of the cultivars.

Negri Alfredo S AS   Prinsi Bhakti B   Failla Osvaldo O   Scienza Attilio A   Espen Luca L  

Frontiers in plant science 20150804


The role of grape berry skin as a protective barrier against damage by physical injuries and pathogen attacks requires a metabolism able to sustain biosynthetic activities such as those relating to secondary compounds (i.e., flavonoids). In order to draw the attention on these biochemical processes, a proteomic and metabolomic comparative analysis was performed among Riesling Italico, Pinot Gris, Pinot Noir, and Croatina cultivars, which are known to accumulate anthocyanins to a different extent  ...[more]

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