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MStern Blot vs. FASP - Different sample types

ABSTRACT: Three different sample types (HeLa lysate, human CSF and human Urine) that were prepared with MStern Blot and FASP. For each sample type there are four replicates.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine, Cerebrospinal Fluid, Cell Culture

SUBMITTER: Sebastian Berger

LAB HEAD: Hanno Steen, PhD

PROVIDER: PXD002572 | Pride | 2015-07-30

REPOSITORIES: Pride

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MStern Blotting-High Throughput Polyvinylidene Fluoride (PVDF) Membrane-Based Proteomic Sample Preparation for 96-Well Plates.

Berger Sebastian T ST Ahmed Saima S Muntel Jan J Cuevas Polo Nerea N Bachur Richard R Kentsis Alex A Steen Judith J Steen Hanno H

Molecular & cellular proteomics : MCP 20150729 10

We describe a 96-well plate compatible membrane-based proteomic sample processing method, which enables the complete processing of 96 samples (or multiples thereof) within a single workday. This method uses a large-pore hydrophobic PVDF membrane that efficiently adsorbs proteins, resulting in fast liquid transfer through the membrane and significantly reduced sample processing times. Low liquid transfer speeds have prevented the useful 96-well plate implementation of FASP as a widely used membra ...[more]

PMID: 26223766

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