Name: chicken deubiquitinases in Salmonella infection
Keywords: Ubiquitin

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Chicken deubiquitinases in Salmonella infection

ABSTRACT: We infected HD11 macrophages with Salmonella Typhimurium, followed by chemical proteomic analysis of deubiquitinases by using ubiquitin-specific active-site probe.

INSTRUMENT(S): LTQ

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Macrophage

DISEASE(S): Salmonella Infectious Disease

SUBMITTER: Mariola Edelmann

LAB HEAD: Mariola J Edelmann

PROVIDER: PXD002594 | Pride | 2018-10-24

REPOSITORIES: Pride

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Activity-Based Proteomic Profiling of Deubiquitinating Enzymes in Salmonella-Infected Macrophages Leads to Identification of Putative Function of UCH-L5 in Inflammasome Regulation.

Kummari Evangel E Alugubelly Navatha N Hsu Chuan-Yu CY Dong Brittany B Nanduri Bindu B Edelmann Mariola J MJ

PloS one 20150812 8

Although protein ubiquitination has been shown to regulate multiple processes during host response to Salmonella enterica serovar Typhimurium infection, specific functions of host deubiquitinating enzymes remain unknown in this bacterial infection. By using chemical proteomics approach, in which deubiquitinating enzymes were labeled by an active-site probe and analyzed by quantitative proteomics, we identified novel deubiquitinases in chicken macrophages based on their reactivity with the probe. ...[more]

PMID: 26267804

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