Isolation of extracellular vesicles by PROSPR

ABSTRACT: Here, we report a novel and inexpensive method of rapidly isolating EVs from small volumes of human blood plasma by PRotein Organic Solvent PRecipitation (PROSPR). PROSPR encompasses a rapid three-step protocol to remove soluble proteins from plasma via precipitation in cold acetone, leaving the lipid-encapsulated EVs behind in suspension. This generates higher purity EVs that can then be obtained from filtration or classical ultracentrifugation methods.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Xavier Gallart Palau  

LAB HEAD: Siu Kwan Sze

PROVIDER: PXD002668 | Pride | 2015-10-05

REPOSITORIES: Pride