Systematic Evaluation of Data-Independent Acquisition for Sensitive and Reproducible Proteomics – a Prototype Design for a Single Injection Assay

ABSTRACT: This dataset includes twelve DIA methods with various mass-isolation windows that analyze precursor ranges from 400-1250 m/z as well as shotgun acquired data with the same instrument and LC gradient. The mass-isolation windows analyzed included 3, 6, and 26 m/z widths. Each 3 m/z window method analyzed a 140 precursor m/z range, 6 m/z windows analyzed either a 200 or 280 precursor m/z range, and the 26 m/z isolation window method analyzed an 800 precursor m/z range from 400-1200 m/z. All data were collected with a 5600+ Sciex QTOF with a 70 minute reverse phase gradient. Shotgun analyses were collected with the sample samples, instrument, and gradient.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Michael Heaven  

LAB HEAD: Kenneth D. Greis

PROVIDER: PXD002688 | Pride | 2015-12-02

REPOSITORIES: Pride