Proteomics,Multiomics

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Systems-level organisation of yeast methylotrophic lifestyle - organelle preparation

ABSTRACT: Changes in the proteome of Pichia pastoris cultures either grown on glucose or methanol were analysed using a 2D-chromatography based TMT labeling LC-ESI-MS workflow. Three biological replicates, with two technical replicates each, had been analyzed leading to six replicate data sets of both growth conditions. The data is supported by peroxisomal protein data.

OTHER RELATED OMICS DATASETS IN: PRJNA280698

INSTRUMENT(S): maXis

ORGANISM(S): Komagataella Pastoris Dsmz 70382

SUBMITTER: Clemens Grünwald-Gruber  

LAB HEAD: Friedrich Altmann

PROVIDER: PXD002831 | Pride | 2015-10-01

REPOSITORIES: Pride

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Publications

<h4>Background</h4>Some yeasts have evolved a methylotrophic lifestyle enabling them to utilize the single carbon compound methanol as a carbon and energy source. Among them, Pichia pastoris (syn. Komagataella sp.) is frequently used for the production of heterologous proteins and also serves as a model organism for organelle research. Our current knowledge of methylotrophic lifestyle mainly derives from sophisticated biochemical studies which identified many key methanol utilization enzymes suc  ...[more]

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