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Characterization of an equine alpha-S2-casein variant

ABSTRACT: Two major forms of equine αS2-casein arising from genomic 1.3 kb in-frame deletion involving two coding exons, one of which represents an equid specific duplication. Findings at the DNA-level have been verified by cDNA sequencing from horse milk of mares with different genotypes. At the protein-level, we were able to show by SDS-page and in-gel digestion with subsequent LC-MS analysis that both proteins are actually expressed

INSTRUMENT(S): Q Exactive

ORGANISM(S): Equus Caballus (horse)

SUBMITTER: Andreas Tholey

LAB HEAD: Andreas Tholey

PROVIDER: PXD002834 | Pride | 2018-10-17

REPOSITORIES: Pride

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Characterization of an Equine α-S2-Casein Variant Due to a 1.3 kb Deletion Spanning Two Coding Exons.

Brinkmann Julia J Koudelka Tomas T Keppler Julia K JK Tholey Andreas A Schwarz Karin K Thaller Georg G Tetens Jens J

PloS one 20151007 10

The production and consumption of mare's milk in Europe has gained importance, mainly based on positive health effects and a lower allergenic potential as compared to cows' milk. The allergenicity of milk is to a certain extent affected by different genetic variants. In classical dairy species, much research has been conducted into the genetic variability of milk proteins, but the knowledge in horses is scarce. Here, we characterize two major forms of equine αS2-casein arising from genomic 1.3 k ...[more]

PMID: 26444874

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