Ontology highlight
ABSTRACT:
INSTRUMENT(S): Synapt G2-S HDMS, TripleTOF 5600
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Embryonic Stem Cell
SUBMITTER: Ellen Scheerlinck
LAB HEAD: Prof. Dr. Dieter Deforce
PROVIDER: PXD002859 | Pride | 2016-07-15
REPOSITORIES: Pride
Proteomics 20160912 20
We present a fully defined culture system (adapted Essential8<sup>TM</sup> [E8<sup>TM</sup> ] medium in combination with vitronectin) for human embryonic stem cells that can be used for SILAC purposes. Although a complete incorporation of the labels was observed after 4 days in culture, over 90% of precursors showed at least 10% conversion. To reduce this arginine conversion, E8<sup>TM</sup> medium was modified by adding (1) l-proline, (2) l-ornithine, (3) N<sup>ω</sup> -hydroxy-nor-l-arginine a ...[more]